Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: GPR84 is a G alpha i protein-coupled receptor that is activated by medium-chain free fatty acids, but only at micromolar concentrations. GPR84 is expressed in leukocytes and its expression is further induced by LPS. 6-OAU is a synthetic agonist for GPR84 (EC50 = 512 nM). It has insignificant actions at a panel of 69 other receptors, channels, or transporters. 6-OAU promotes chemotaxis in human neutrophils (EC50 = 318 nM) and amplifies TNF- alpha production by LPS-treated U937 macrophages. In rats, 6-OAU (1 mg/ml) drives the recruitment of neutrophils and macrophages when injected into a subcutaneous air pouch and raises serum levels of CXCL1 (growth-regulated alpha protein) when delivered intravenously.
6-OAU is supplied as a crystalline solid. A stock solution may be made by dissolving the 6-OAU in the solvent of choice. 6-OAU is soluble in organic solvents such DMSO and dimethyl formamide, which should be purged with an inert gas. The solubility of 6-OAU in these solvents is approximately 0.25 mg/ml. 6-OAU is sparingly soluble in aqueous solutions. To enhance aqueous solubility, dilute the organic solvent solution into aqueous buffers or isotonic saline. If performing biological experiments, ensure the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. We do not recommend storing the aqueous solution for more than one day.
Davenport, A.P., Alexander, S.P.H., Sharman, J.L., et al. International Union of Basic and Clinical Pharmacology. LXXXVIII. G protein-coupled receptor list: Recommendations for new pairings with cognate ligands. Pharmacol. Rev. 65(3), 967-986 (2013).
Suzuki, M., Takaishi, S., Nagasaki, M., et al. Medium-chain fatty acid-sensing receptor, GPR84, is a proinflammatory receptor. J. Biol. Chem. 288(15), 10684-10691 (2013). O N H N H O N H Product Information Cayman Chemical Mailing address 1180 E. Ellsworth Road Ann Arbor, MI 48108 USA Phone (800) 364-9897 (734) 971-3335 Fax (734) 971-3640 E-Mail email@example.com Web www.caymanchem.com