Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
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TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: 2-Ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine is a nonpeptidic angiotensin II receptor antagonist whose imidazo[4,5-b]pyridine scaffold has been used to examine a variety of positional substitutions in the development of orally active, long duration antihypertensive agents.
2-Ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine is supplied as a crystalline solid. A stock solution may be made by dissolving the 2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine in the solvent of choice. 2-Ethyl-5,7-dimethyl-3Himidazo[4,5-b]pyridine is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of 2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine in these solvents is approximately 10, 20, and 30 mg/ml, respectively. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. 2-Ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine is sparingly soluble in aqueous buffers. The solubility of 2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine in PBS, pH 7.2, is approximately 0.25 mg/ml. For maximum solubility in aqueous buffers, 2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine should first be dissolved in DMF and then diluted with the aqueous buffer of choice. 2-Ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Mantlo, N.B., Chakravarty, P.K., Ondeyka, D.L. , et al. Potent, orally active Imadazo[4,5-b]pyridine-based angiotensin II receptor antagonists. J. Med. Chem. 34(9), 2919-2922 (1991).