Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: 1,25-dihydroxy Vitamin D2 is the active circulating metabolite of vitamin D2 formed by 25-hydroxylase in the liver and 25-hydroxyvitamin D-hydroxylase in the kidney. By binding to the vitamin D receptor (VDR), 1,25-dihydroxy Vitamin D2 activates VDR to interact with the retinoid X receptor (RXR), forming the VDR/RXR/cofactor complex, which regulates gene transcription. Because 1,25-dihydroxy Vitamin D2 circulates at picomolar concentrations and is highly lipophilic, it is difficult to quantify.
Ercalcitriol is supplied as a crystalline solid. A stock solution may be made by dissolving the ercalcitriol in the solvent of choice. Ercalcitriol is soluble in ethanol, which should be purged with an inert gas. The solubility of ercalcitriol in ethanol is approximately 1 mg/ml. Ercalcitriol is sparingly soluble in aqueous solutions. To enhance aqueous solubility, dilute the organic solvent solution into aqueous buffers or isotonic saline. If performing biological experiments, ensure the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. We do not recommend storing the aqueous solution for more than one day.
Sudsakorn, S., Phatarphekar, A., O’Shea, T., et al. Determination of 1,25-dihydroxyvitamin D2 in rat serum using liquid chromatography with tandem mass spectrometry. J. Chromatogr. B 879, 139-145 (2011).
Kato, S., Kim, M.-S., Yamaoka, K., et al. Mechanisms of transcriptional repression by 1,25(OH)2 vitamin D. Curr. Opin. Nephrol. Hypertens. 16, 297-304 (2007).