Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Vitamin D aids in the absorption of calcium and has central roles in bone formation and maintenance, hypertension, cancer, and immunity. In addition, the active form of vitamin D, 1,25-dihydroxy vitamin D3, decreases the proliferation and increases differentiation of keratinocytes. 1,24-dihydroxy Vitamin D3 is an analog of 1,25-dihydroxy vitamin D3 which, when used in a 4 µg/g ointment, safely and effectively suppresses psoriasis. It also is effective at increasing the time interval between phototherapy cycles when ultraviolet light is used for psoriasis. 1,24-dihydroxy Vitamin D3 inhibits the growth of both normal and psoriatic human keratinocytes (IC50 ~ 10 nM) via the vitamin D receptor. It also suppresses the production of RANTES and IL-8 by human dermal fibroblasts and reduces cutaneous lymphocyte-associated antigen on CD4+ T cells, impairing migration and skin infiltration.<sup class="reference
1,24-dihydroxy Vitamin D3 is supplied as a crystalline solid. A stock solution may be made by dissolving the 1,24-dihydroxy vitamin D3 in the solvent of choice. 1,24-dihydroxy Vitamin D3 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. The solubility of 1,24-dihydroxy vitamin D3 in these solvents is approximately 20 mg/ml. 1,24-dihydroxy Vitamin D3 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, 1,24-dihydroxy vitamin D3 should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. 1,24-dihydroxy Vitamin D3 has a solubility of approximately 0.3 mg/ml in a 1:3 solution of ethanol:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
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