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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

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Universal Kinase Activity Kit (Fluorometric) - LS-K374
Available for shipment within the USA only
Assay Kit
Catalog
Size
Price
LS-K374-100
100 asy
$515

Available for shipment within the USA only

Product Description

Protein Kinases belong to a large family of phosphotransferases that mediate a wide spectrum of metabolic events via signal transduction pathways. Protein Kinases catalyze transfer of a phosphate group from a phosphate donor to a substrate protein. Kinases modulate the function of numerous proteins and are well-known therapeutic targets in many diseases like cancer, inflammation and diabetes etc. The ADPsensor Universal Kinase Assay Kit is based on the fluorescence detection of ADP generated by Kinase activity. In this assay, ADP formed during the kinase reaction is converted to an intermediate by the ADP Sensor Mix, which then reacts with a probe to generate a strong and stable fluorescence signal at Ex/Em = 535/587 nm. ATP in the kinase reactions doesn't interfere with the assay; therefore our kinase assay can be read kinetically (or at endpoint). The kit provides a universal tool to measure kinase activity in presence of substrates of your interest, or to screen/characterize kinase inhibitors. The assay is simple, non-radioactive, highly sensitive (<0.05 µM ADP) with broad ATP tolerance (up to 1 mM). The kit can detect as low as 1 ng of active Kinase.

Specifications

Name
Universal Kinase Activity Kit (Fluorometric)
Product Type
Enzymatic Activity Assay
Usage Summary
Detection of Kinase activity, Characterization of Kinase substrates and inhibitors.
Target
Kinase
Sample Type
Crude Cell Extract, Crude Tissue Extract, Immuno-precipitated Kinases, Purified Kinases, Semi-purified Kinases
Detection
Fluorometric (Ex/Em 535/587 nm)
Species Reactivity
All species
Kit Components
  • Kinase Assay Buffer
  • ADP Sensor I
  • ADP Sensor II
  • Probe (in DMSO)
  • Ultra-pure ATP (ADP depleted)
  • DTT (1M)
  • ADP Standard
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C for up to 1 year., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Requested From: United States
    Date Requested: 12/10/2018
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