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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Superoxide Dismutase (SOD) Activity Kit (Color) - LS-K25

Available for shipment within the USA only
Catalog
Size
Price
LS-K25-100
100 asy
$440

Available for shipment within the USA only

Product Description

Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the super oxide anion into hydrogen peroxide and molecular oxygen. The sensitive SOD assay kit utilizes WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anionis linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD (below). Therefore, the inhibition activity of SOD can be determined by a colorimetric method.

Specifications

Name
Superoxide Dismutase (SOD) Activity Kit (Color)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Superoxide Dismutase (SOD) activity in various biological sample types.
Target
Superoxide Dismutase
Sample Type
Biological Fluids, Cell Culture Media, Cell Lysates, Plasma, Serum, Tissue Homogenates, Urine
Detection
Colorimetric (450 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • WST Solution
  • SOD Enzyme Solution
  • SOD Assay Buffer
  • SOD Dilution Buffer
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped +4°C Ice Packs, Store at Store at 4°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Assay Result

    SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.
    SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.

    Assay Result

    SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.
    SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.

    Assay Result

    SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.
    SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.

    Assay Result

    SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.
    SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.

    Requested From: United States
    Date Requested: 4/23/2019
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