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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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LSBio
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Catalog Number Size Price
LS-K241-5000 5000 tst $195 
LS-K241-10000 10000 tst $310 
Cell Viability Assay Kit

Resorufin Blue Cell Viability Assay Kit (Fluorometric) - LS-K241

Resorufin Blue Cell Viability Assay Kit (Fluorometric) - LS-K241

Available for shipment within the USA only
Description:
This homogeneous assay involves simply adding a single reagent, the reagent, to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 - 570 nm, emission wavelength = 590 - 620 nm) after an incubation step. The reagent, like other resazurin-based assays such as the Alamar Blue reagent, utilizes the redox dye resazurin which is not fluorescent, but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a fluorescence spectrophotometer or plate reader. Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The reagent has been optimized for maximum sensitivity, reproducibility and long shelf-life. The homogeneous cell-based assay can be performed in multi-well plates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Applications include cell proliferation, cytotoxicity and apoptosis.
Price
Catalog Number
$195 
LS-K241-5000

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
This homogeneous assay involves simply adding a single reagent, the reagent, to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 - 570 nm, emission wavelength = 590 - 620 nm) after an incubation step. The reagent, like other resazurin-based assays such as the Alamar Blue reagent, utilizes the redox dye resazurin which is not fluorescent, but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a fluorescence spectrophotometer or plate reader. Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The reagent has been optimized for maximum sensitivity, reproducibility and long shelf-life. The homogeneous cell-based assay can be performed in multi-well plates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Applications include cell proliferation, cytotoxicity and apoptosis.

Specifications

Name
Resorufin Blue Cell Viability Assay Kit (Fluorometric)
Type
Cell Viability
Usage
Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. As low as 100 cells can be accurately quantified.
Target
Cell Viability
SampleType
Cell Cultures
Detection
Fluorometric (Ex/Em 530/590 nm)
Components
  • Reagent
  • Control Reagent
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped Ambient, Store at 4°C, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee

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    Fluorimetry

    Cell Viability Assay Kit

    Fluorimetry

    Cell Viability Assay Kit

    Fluorimetry

    Cell Viability Assay Kit

    Fluorimetry

    Cell Viability Assay Kit

    Requested From: United States
    Date Requested: 2/26/2020