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Nitric Oxide Synthase (NOS) Inhibitor Screening Kit (Fluor) - LS-K102

Available for shipment within the USA only
Catalog
Size
Price
LS-K102-100
100 asy
$500

Available for shipment within the USA only

Product Description

Nitric oxide synthases (EC 1.14.13.39) (NOS) is a family of enzymes that catalyze the production of nitric oxide (NO). In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. Nitric oxide plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In contrast to its beneficial effects, NO has also been associated with numerous pathological situations such as hypotension accompanying septic shock, essential hypertension, and atherosclerosis. The overproduction of NO has been found to be the fundamental cause underlying neurodegenerative disorders and neuropathic pain. Therefore, developing small molecules for inhibition of NOS is therapeutically desirable. LSBio's Nitric Oxide Synthase Inhibitor Screening Kit provides a simple and high-throughput adaptable method to screen/study/characterize potential NOS inhibitors. In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with the fluorescent probe to generate a stable signal at Ex/Em = 360/450 nm, which is directly proportional to NOS activity. In the presence of a NOS-specific inhibitor, the formation of NO is reduced/abolished resulting in decrease or total loss of the fluorescence.

Specifications

Name
Nitric Oxide Synthase (NOS) Inhibitor Screening Kit (Fluor)
Product Type
Inhibitor Screening
Usage Summary
This assay provides for the detection of NOS activity, specifically screening/studying/characterizing potential NOS inhibitors.
Target
NOS / Nitric Oxide Synthase
Sample Type
Small Molecules
Detection
Fluorometric (Ex/Em 360/450 nm)
Kit Components
  • NOS Assay Buffer
  • NOS Dilution Buffer
  • NOS Enzyme
  • NOS Inhibitor (DPI 1 mM)
  • NOS Substrate
  • NOS Cofactor 1
  • NOS Cofactor 2 (25X)
  • Enhancer
  • Nitrate Reductase
  • Probe
  • NaOH
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -80°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    Inhibition of NOS Enzyme activity by Diphenyleneiodonium chloride. IC50 of Diphenyleneiodonium chloride was determined to be 20 nM.
    Inhibition of NOS Enzyme activity by Diphenyleneiodonium chloride. IC50 of Diphenyleneiodonium chloride was determined to be 20 nM.

    Assay Result

    Inhibition of NOS Enzyme activity by Diphenyleneiodonium chloride. IC50 of Diphenyleneiodonium chloride was determined to be 20 nM.
    Inhibition of NOS Enzyme activity by Diphenyleneiodonium chloride. IC50 of Diphenyleneiodonium chloride was determined to be 20 nM.

    Assay Result

    Inhibition of NOS Enzyme activity by Diphenyleneiodonium chloride. IC50 of Diphenyleneiodonium chloride was determined to be 20 nM.
    Inhibition of NOS Enzyme activity by Diphenyleneiodonium chloride. IC50 of Diphenyleneiodonium chloride was determined to be 20 nM.

    Assay Result

    Inhibition of NOS Enzyme activity by Diphenyleneiodonium chloride. IC50 of Diphenyleneiodonium chloride was determined to be 20 nM.
    Inhibition of NOS Enzyme activity by Diphenyleneiodonium chloride. IC50 of Diphenyleneiodonium chloride was determined to be 20 nM.

    Requested From: United States
    Date Requested: 3/26/2019
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