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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Nitric Oxide Synthase (NOS) Assay Kit (Colorimetric) - LS-K196

Available for shipment within the USA only
Assay Kit
Catalog
Size
Price
LS-K196-100
100 tst
$395

Available for shipment within the USA only

Product Description

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Simple, direct and non-radioactive procedures for measuring NOS are becoming popular in Research and Drug Discovery. This Nitric Oxide Synthase Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess Method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 40 min.

Specifications

Name
Nitric Oxide Synthase (NOS) Assay Kit (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor.
Target
NOS / Nitric Oxide Synthase
Sample Type
Biological Samples
Detection
Colorimetric (540 nm Absorbance)
Kit Components
  • Assay Buffer
  • Substrate
  • GDH
  • Reagent A
  • Reagent B
  • Reagent C
  • Reagent D: Dried Reagent E
  • ZnSO 4
  • standard NaOH
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible), Spectrophotometer (visible)
    Conditions
    Shipped +4°C Ice Packs, Store at Store at 4°C or -20°C., 3 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Requested From: United States
    Date Requested: 7/19/2019
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