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Nitric Oxide Synthase (NOS) Activity Kit (Fluor) - LS-K53

Available for shipment within the USA only
Catalog
Size
Price
LS-K53-100
100 asy
$500

Available for shipment within the USA only

Product Description

Nitric oxide synthases (EC 1.14.13.39) (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidinonitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS),neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body's defense mechanism. LSBio's Nitric Oxide Synthase Activity Assay Kit provides an accurate and convenient method to assay NOS activity in a variety of samples. In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with the fluorescent probe to generate a stable signal at Ex/Em = 360/450 nm, which is directly proportional to NOS activity. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 0.75 µU of NOS activity.

Specifications

Name
Nitric Oxide Synthase (NOS) Activity Kit (Fluor)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Nitric oxide synthases (NOS) activity in purified protein, tissues, and cells.
Target
NOS / Nitric Oxide Synthase
Sample Type
Cell Extracts, Purified Recombinant Protein, Tissue Extracts
Detection
Fluorometric (Ex/Em 360/450 nm)
Species Reactivity
Mammal
Kit Components
  • NOS Assay Buffer
  • NOS Dilution Buffer
  • NOS Substrate
  • NOS Cofactor 1
  • NOS Cofactor 2 (25X)
  • Nitrate Reductase
  • NOS (Positive Control)
  • Enhancer
  • Nitrite Standard
  • Probe 1
  • NaOH 2
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -80°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    (a) Detection of endogenous NOS activity in135 µg of J774 and 157 µg of HeLa cells and (b) detection of endogenous NOS activity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.
    (a) Detection of endogenous NOS activity in135 µg of J774 and 157 µg of HeLa cells and (b) detection of endogenous NOS activity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.

    Assay Result

    (a) Detection of endogenous NOS activity in135 µg of J774 and 157 µg of HeLa cells and (b) detection of endogenous NOS activity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.
    (a) Detection of endogenous NOS activity in135 µg of J774 and 157 µg of HeLa cells and (b) detection of endogenous NOS activity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.

    Assay Result

    (a) Detection of endogenous NOS activity in135 µg of J774 and 157 µg of HeLa cells and (b) detection of endogenous NOS activity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.
    (a) Detection of endogenous NOS activity in135 µg of J774 and 157 µg of HeLa cells and (b) detection of endogenous NOS activity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.

    Assay Result

    (a) Detection of endogenous NOS activity in135 µg of J774 and 157 µg of HeLa cells and (b) detection of endogenous NOS activity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.
    (a) Detection of endogenous NOS activity in135 µg of J774 and 157 µg of HeLa cells and (b) detection of endogenous NOS activity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.

    Requested From: United States
    Date Requested: 7/19/2019
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