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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Neutral Red Cell Cytotoxicity Assay Kit (Colorimetric) - LS-K490

Available for shipment within the USA only
Catalog
Size
Price
LS-K490-1000
1000 asy
$385

Available for shipment within the USA only

Product Description

Neutral red cell cytotoxicity assay is one of the common methods used to detect cell viability or drug cytotoxicity. The principle of this assay is based on the detection of viable cells via the uptake of the dye neutral red. Neutral red is a eurhodin dye that stains lysosomes in viable cells. Viable cells can take up neutral red via active transport and incorporate the dye into their lysosomes but non-viable cells cannot not take up this chromophore. Consequently, after washing, viable cells can release the incorporated dye in under acidified-extracted conditions. The amount of released dye can be used to determine the total number of viable cells or drug cytotoxicity. The neutral red uptake assay provides a quantitative measurement of the number of viable cells and can be measured at OD 540 nm. The Neutral Red Cell Cytotoxicity Assay Kit is simple, accurate, and reproducible. It also includes Doxorubicin, as a positive control. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening that can detect between 5,000-50,000 cells per well.

Specifications

Name
Neutral Red Cell Cytotoxicity Assay Kit (Colorimetric)
Product Type
Cytotoxicity Assay
Usage Summary
This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of neutral cells to stain lysosomes of viable cells.
Target
Cell Viability
Sample Type
Adherent Cells, Non-adherent Cells
Detection
Colorimetric (540 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • 100X Neutral Red Staining Solution
  • 10X Washing Solution
  • 2X Solubilization Solution
  • 20 mM Doxorubicin
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C for up to 1 year, 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    (a) Dose-response curve of HepG2 and (b) MCF-7 cells to doxorubicin for 72 hours determined by the Neutral Red Cell Cytotoxicity assay. Assays were performed according to the kit protocol in triplicate.
    (a) Dose-response curve of HepG2 and (b) MCF-7 cells to doxorubicin for 72 hours determined by the Neutral Red Cell Cytotoxicity assay. Assays were performed according to the kit protocol in triplicate.

    Assay Result

    (a) Dose-response curve of HepG2 and (b) MCF-7 cells to doxorubicin for 72 hours determined by the Neutral Red Cell Cytotoxicity assay. Assays were performed according to the kit protocol in triplicate.
    (a) Dose-response curve of HepG2 and (b) MCF-7 cells to doxorubicin for 72 hours determined by the Neutral Red Cell Cytotoxicity assay. Assays were performed according to the kit protocol in triplicate.

    Assay Result

    (a) Dose-response curve of HepG2 and (b) MCF-7 cells to doxorubicin for 72 hours determined by the Neutral Red Cell Cytotoxicity assay. Assays were performed according to the kit protocol in triplicate.
    (a) Dose-response curve of HepG2 and (b) MCF-7 cells to doxorubicin for 72 hours determined by the Neutral Red Cell Cytotoxicity assay. Assays were performed according to the kit protocol in triplicate.

    Assay Result

    (a) Dose-response curve of HepG2 and (b) MCF-7 cells to doxorubicin for 72 hours determined by the Neutral Red Cell Cytotoxicity assay. Assays were performed according to the kit protocol in triplicate.
    (a) Dose-response curve of HepG2 and (b) MCF-7 cells to doxorubicin for 72 hours determined by the Neutral Red Cell Cytotoxicity assay. Assays were performed according to the kit protocol in triplicate.

    Requested From: United States
    Date Requested: 9/15/2019