Maltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a a-1,4-glycosidic linkage, which can be easily converted to two glucoses by a-D-glucosidase. The generated glucose can be specifically oxidized to produce a product that interacts with the probe to generate color and fluorescence. Thus, maltose can be determined by either colorimetric (spectrophotometry at ? = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The kit provides a fast, easy and sensitive method for quantifying maltose in various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.).