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Lysosomal Cytotox Dual Staining Kit (Cell-Based)(Fluor) - LS-K496

Available for shipment within the USA only
Catalog
Size
Price
LS-K496-50
50 asy
$665

Available for shipment within the USA only

Product Description

Lysosomes are membrane-bound organelles important for various cellular processes. They contain hydrolytic enzymes utilized in the metabolism of some biomolecules. The extracellular cargo (e.g. nutrients, toxins) binds to the cell membrane and is subsequently transported into membrane-bound endosomes for further degradation by lysosomes while intracellular components are transported to lysosomes through autophagy. Lysosomal dysfunction is associated with many human conditions such as aging and neurodegenerative disease. has developed the Lysosomal Cytotoxicity Dual Staining Kit (cell-based) which contains two probes; membrane permeable, selective Lysosomal Stain and Cell Death Marker. We also include a Positive Control Reagent, which increases lysosome activity and staining, thus serves as an experimental control. This easy-to-use, non-radioactive kit allows studying the regulation of lysosome and cytotoxicity at the cellular level by using Fluorescence Microscopy and Flow Cytometry in cultured cells.

Specifications

Name
Lysosomal Cytotox Dual Staining Kit (Cell-Based)(Fluor)
Product Type
Cytotoxicity Assay
Usage Summary
Measurement of lysosomal cytotoxicity. . Staining for lysosome cytotoxicity and cell death.. Screening for compounds that affect lysosomal function
Target
Cell Viability
Sample Type
Adherent Cells, Suspension Cells
Detection
Flow Cytometry (FL1/FL2)
Species Reactivity
Mammal
Kit Components
  • Assay Buffer (10X)
  • Lysosomal Stain (500X)
  • Cell Death Marker (500X)
  • Positive Control Reagent
  • (See Datasheet for specific volumes supplied)
  • Applications
    Flow Cytometry, Microscopy (fluorescent)
    Equipment
    Flow cytometer, Fluorescence microscope
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C for up to 1 year, 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

    Publications (0)


    Customer Reviews (0)


    Images

    Fluorescence Microscopy

    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.
    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.

    Fluorescence Microscopy

    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.
    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.

    Fluorescence Microscopy

    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.
    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.

    Fluorescence Microscopy

    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.
    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.

    Requested From: United States
    Date Requested: 9/15/2019