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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-K496-50 50 asy $605 
Cell Viability Assay Kit - Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.

Lysosomal Cytotox Dual Staining Kit (Cell-Based)(Fluor) - LS-K496

Lysosomal Cytotox Dual Staining Kit (Cell-Based)(Fluor) - LS-K496

Available for shipment within the USA only
Description:
Lysosomes are membrane-bound organelles important for various cellular processes. They contain hydrolytic enzymes utilized in the metabolism of some biomolecules. The extracellular cargo (e.g. nutrients, toxins) binds to the cell membrane and is subsequently transported into membrane-bound endosomes for further degradation by lysosomes while intracellular components are transported to lysosomes through autophagy. Lysosomal dysfunction is associated with many human conditions such as aging and neurodegenerative disease. has developed the Lysosomal Cytotoxicity Dual Staining Kit (cell-based) which contains two probes; membrane permeable, selective Lysosomal Stain and Cell Death Marker. We also include a Positive Control Reagent, which increases lysosome activity and staining, thus serves as an experimental control. This easy-to-use, non-radioactive kit allows studying the regulation of lysosome and cytotoxicity at the cellular level by using Fluorescence Microscopy and Flow Cytometry in cultured cells.
Price
Catalog Number
$605 
LS-K496-50
50 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
Lysosomes are membrane-bound organelles important for various cellular processes. They contain hydrolytic enzymes utilized in the metabolism of some biomolecules. The extracellular cargo (e.g. nutrients, toxins) binds to the cell membrane and is subsequently transported into membrane-bound endosomes for further degradation by lysosomes while intracellular components are transported to lysosomes through autophagy. Lysosomal dysfunction is associated with many human conditions such as aging and neurodegenerative disease. has developed the Lysosomal Cytotoxicity Dual Staining Kit (cell-based) which contains two probes; membrane permeable, selective Lysosomal Stain and Cell Death Marker. We also include a Positive Control Reagent, which increases lysosome activity and staining, thus serves as an experimental control. This easy-to-use, non-radioactive kit allows studying the regulation of lysosome and cytotoxicity at the cellular level by using Fluorescence Microscopy and Flow Cytometry in cultured cells.

Specifications

Name
Lysosomal Cytotox Dual Staining Kit (Cell-Based)(Fluor)
Type
Cytotoxicity Assay
Usage
Measurement of lysosomal cytotoxicity. . Staining for lysosome cytotoxicity and cell death.. Screening for compounds that affect lysosomal function
Target
Cell Viability
SampleType
Adherent Cells, Suspension Cells
Detection
Flow Cytometry (FL1/FL2)
Species Reactivity
Mammal
Components
  • Assay Buffer (10X)
  • Lysosomal Stain (500X)
  • Cell Death Marker (500X)
  • Positive Control Reagent
  • (See Datasheet for specific volumes supplied)
  • Applications
    Flow Cytometry, Microscopy (fluorescent)
    Equipment
    Flow cytometer, Fluorescence microscope
    Conditions
    Shipped -20°C Dry Ice, Components stored at varying temperatures. See recommended storage details in product insert, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Measurement of lysosomal cytotoxicity

    Publications (0)

    Customer Reviews (0)

    Images

    Fluorescence Microscopy

    Cell Viability Assay Kit - Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.
    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.

    Fluorescence Microscopy

    Cell Viability Assay Kit - Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.
    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.

    Fluorescence Microscopy

    Cell Viability Assay Kit - Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.
    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.

    Fluorescence Microscopy

    Cell Viability Assay Kit - Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.
    Lysosomal Staining in Jurkat cells. 1x106 Jurkat cells were treated with vehicle or Positive Control Reagent for 24 hours. After treatment, cells were incubated in 1 ml of Assay Buffer with 1X Lysosomal Staining for 10 minutes at 37°C, followed by stain with 1X Cell Death Marker according to the kit’s protocol. Cells treated with vehicle (left panel; basal level of lysosomal staining), or Positive Control Reagent (right panel; green fluorescence). Increased green fluorescence compared to the basal levels confirms accumulation of Lysosomal Stain inside activated lysosomes. Low levels of red fluorescence resulting from Cell Death Marker shows low levels of Lysosomal Stain toxicity.

    Requested From: United States
    Date Requested: 7/4/2020