Products
Services
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-K528-100 100 asy $480 
Cell Viability Assay Kit - Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.

Live/Dead Mammalian Cell Viability Assay Kit (Fluorometric) - LS-K528

Live/Dead Mammalian Cell Viability Assay Kit (Fluorometric) - LS-K528

Available for shipment within the USA only
Description:
Quantification of number of live and dead cells is an indispensable tool in cell biology research. The Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact, live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
Price
Catalog Number
$480 
LS-K528-100
100 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
Quantification of number of live and dead cells is an indispensable tool in cell biology research. The Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact, live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.

Specifications

Name
Live/Dead Mammalian Cell Viability Assay Kit (Fluorometric)
Type
Cell Viability
Usage
Screening/studying/characterization of stimulators/inhibitors that affect cell viability.
Target
Cell Viability
SampleType
Adherent Cells, Suspension Cells
Detection
Fluorescent or FACS analysis (Ex/Em = 485/530 nm) to detect stained live cells; Fluorescent or FACS analysis (Ex/Em = 495/635 nm) to detect stained dead cells
Species Reactivity
Mammal
Components
  • Assay Buffer
  • Live Cell Staining Dye
  • Dead Cell Staining Dye
  • (See Datasheet for specific volumes supplied)
  • Applications
    Flow Cytometry, Microscopy (fluorescent)
    Equipment
    Flow cytometer, Fluorescence microscope, Microscope (visible)
    Conditions
    Shipped -20°C Dry Ice, Components stored at varying temperatures. See recommended storage details in product insert, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Easy to use
    • Measures cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis
    • Non-radioactive detection: Detects using either microscopy or flow cytometry

    Publications (0)

    Customer Reviews (0)

    Images

    Microscopy

    Cell Viability Assay Kit - Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.
    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.

    Microscopy

    Cell Viability Assay Kit - Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.
    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.

    Microscopy

    Cell Viability Assay Kit - Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.
    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.

    Microscopy

    Cell Viability Assay Kit - Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.
    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.

    Requested From: United States
    Date Requested: 7/11/2020