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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Live/Dead Mammalian Cell Viability Assay Kit (Fluorometric) - LS-K528

Available for shipment within the USA only
Catalog
Size
Price
LS-K528-100
100 asy
$515

Available for shipment within the USA only

Product Description

Quantification of number of live and dead cells is an indispensable tool in cell biology research. The Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact, live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.

Specifications

Name
Live/Dead Mammalian Cell Viability Assay Kit (Fluorometric)
Product Type
Cell Viability
Usage Summary
Screening/studying/characterization of stimulators/inhibitors that affect cell viability.
Target
Cell Viability
Sample Type
Adherent Cells, Suspension Cells
Detection
Fluorescent or FACS analysis (Ex/Em = 485/530 nm) to detect stained live cells; Fluorescent or FACS analysis (Ex/Em = 495/635 nm) to detect stained dead cells
Species Reactivity
Mammal
Kit Components
  • Assay Buffer
  • Live Cell Staining Dye
  • Dead Cell Staining Dye
  • (See Datasheet for specific volumes supplied)
  • Applications
    Flow Cytometry, Microscopy (fluorescent)
    Equipment
    Flow cytometer, Fluorescence microscope, Microscope (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C for up to 1 year, 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

    Publications (0)


    Customer Reviews (0)


    Images

    Microscopy

    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.
    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.

    Microscopy

    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.
    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.

    Microscopy

    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.
    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.

    Microscopy

    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.
    Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 µM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.

    Requested From: United States
    Date Requested: 9/15/2019