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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Lactose Assay Kit (Colorimetric/Fluorometric) - LS-K128
Available for shipment within the USA only
Catalog
Size
Price
LS-K128-100
100 asy
$500

Available for shipment within the USA only

Product Description

Lactose (C12H22O11 FW: 342.3) is an important naturally occurred disaccharide, consisting of one galactose and one glucose. Milk contains ~2-8% lactose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage. In the LSBio Lactose Assay Kit, Lactose is hydrolyzed to glucose and galactose. The galactose is subsequently oxidized generating color (O.D 570 nm) and fluorescence (Ex/Em 535/587 nm). Free galactose can be corrected by a background control in the absence of lactase. The Lactose Assay Kit provides a simple, convenient, and sensitive means for direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). Pretreatment of samples is not required. The kit can be used as a high throughput assay.

Specifications

Name
Lactose Assay Kit (Colorimetric/Fluorometric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection and measurement of Lactose in various biological samples.
Target
Lactose
Sample Type
Biological Fluids, Food and Beverages, Plasma, Serum, Tissue Culture Supernatants, Urine
Detection
Colorimetric (570 nm Absorbance), Fluorometric (Ex/Em 535/587 nm)
Species Reactivity
Mammal
Kit Components
  • Lactose Assay Buffer
  • Probe (DMSO solution)
  • Lactase (Lyophilized)
  • Lactose Enzyme Mix (Lyophilized)
  • HRP (Lyophilized)
  • Lactose Standard (100 nmol/¦l)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric), Spectrophotometry (visible)
    Equipment
    Microplate fluorometer, Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Customer Reviews (0)


    Images

    Assay Result

    Lactose Standard Curve with galactose control. The kit detected galactose and lactose equally. In the absence of Lactase, the kit detected galactose, but not lactose.
    Lactose Standard Curve with galactose control. The kit detected galactose and lactose equally. In the absence of Lactase, the kit detected galactose, but not lactose.

    Assay Result

    Lactose Standard Curve with galactose control. The kit detected galactose and lactose equally. In the absence of Lactase, the kit detected galactose, but not lactose.
    Lactose Standard Curve with galactose control. The kit detected galactose and lactose equally. In the absence of Lactase, the kit detected galactose, but not lactose.

    Assay Result

    Lactose Standard Curve with galactose control. The kit detected galactose and lactose equally. In the absence of Lactase, the kit detected galactose, but not lactose.
    Lactose Standard Curve with galactose control. The kit detected galactose and lactose equally. In the absence of Lactase, the kit detected galactose, but not lactose.

    Assay Result

    Lactose Standard Curve with galactose control. The kit detected galactose and lactose equally. In the absence of Lactase, the kit detected galactose, but not lactose.
    Lactose Standard Curve with galactose control. The kit detected galactose and lactose equally. In the absence of Lactase, the kit detected galactose, but not lactose.

    Requested From: United States
    Date Requested: 12/13/2018
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