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Intracellular NOS Detection Kit (Fluorometric) - LS-K101

Available for shipment within the USA only
Catalog
Size
Price
LS-K101-100
100 asy
$400

Available for shipment within the USA only

Product Description

Nitric oxide synthases (EC 1.14.13.39) (NOSs) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved with the regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body's defense mechanism. LSBio's Intracellular Nitric Oxide Synthase Detection kit uses a dye that reacts with intracellular NO produced by NOS to produce fluorescence (Ex/Em = 485/530 nm), which is proportional to the concentration of intracellular NOS and can be detected using a microplate reader or a fluorescence microscope. This kit provides a simple, non-radiometric method for detection of intracellular NOS in the cells.

Specifications

Name
Intracellular NOS Detection Kit (Fluorometric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection of NOS activity in adherent cells, and the screening/studying/characterizing stimulators/inhibitors that affect intracellular levels of NOS.
Target
NOS / Nitric Oxide Synthase
Sample Type
Cells
Detection
Fluorometric (Ex/Em 485/530 nm)
Species Reactivity
Mammal
Kit Components
  • NOS Assay Buffer
  • Staining Dye (In DMSO)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Microscopy (fluorescent), Spectrophotometry (fluorometric)
    Equipment
    Fluorescence microscope, Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Customer Reviews (0)


    Images

    Assay Result

    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. A) Detection with a plate reader: The fluorescence signal was measured at Ex/Em = 485/530 nm. Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.
    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. A) Detection with a plate reader: The fluorescence signal was measured at Ex/Em = 485/530 nm. Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.

    Microscopy

    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. Detection with a fluorescent microscope: The cells were washed with Assay buffer and imaged using Nikon TiE microscope. (a) and (a) control cells (vehicle treated) (c) and (d) cells treated with LPS (200 ng/ml) and ?-INF (100 ng/ml). Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.
    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. Detection with a fluorescent microscope: The cells were washed with Assay buffer and imaged using Nikon TiE microscope. (a) and (a) control cells (vehicle treated) (c) and (d) cells treated with LPS (200 ng/ml) and ?-INF (100 ng/ml). Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.

    Assay Result

    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. A) Detection with a plate reader: The fluorescence signal was measured at Ex/Em = 485/530 nm. Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.
    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. A) Detection with a plate reader: The fluorescence signal was measured at Ex/Em = 485/530 nm. Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.

    Microscopy

    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. Detection with a fluorescent microscope: The cells were washed with Assay buffer and imaged using Nikon TiE microscope. (a) and (a) control cells (vehicle treated) (c) and (d) cells treated with LPS (200 ng/ml) and ?-INF (100 ng/ml). Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.
    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. Detection with a fluorescent microscope: The cells were washed with Assay buffer and imaged using Nikon TiE microscope. (a) and (a) control cells (vehicle treated) (c) and (d) cells treated with LPS (200 ng/ml) and ?-INF (100 ng/ml). Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.

    Assay Result

    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. A) Detection with a plate reader: The fluorescence signal was measured at Ex/Em = 485/530 nm. Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.
    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. A) Detection with a plate reader: The fluorescence signal was measured at Ex/Em = 485/530 nm. Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.

    Microscopy

    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. Detection with a fluorescent microscope: The cells were washed with Assay buffer and imaged using Nikon TiE microscope. (a) and (a) control cells (vehicle treated) (c) and (d) cells treated with LPS (200 ng/ml) and ?-INF (100 ng/ml). Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.
    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. Detection with a fluorescent microscope: The cells were washed with Assay buffer and imaged using Nikon TiE microscope. (a) and (a) control cells (vehicle treated) (c) and (d) cells treated with LPS (200 ng/ml) and ?-INF (100 ng/ml). Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.

    Assay Result

    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. A) Detection with a plate reader: The fluorescence signal was measured at Ex/Em = 485/530 nm. Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.
    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. A) Detection with a plate reader: The fluorescence signal was measured at Ex/Em = 485/530 nm. Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.

    Microscopy

    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. Detection with a fluorescent microscope: The cells were washed with Assay buffer and imaged using Nikon TiE microscope. (a) and (a) control cells (vehicle treated) (c) and (d) cells treated with LPS (200 ng/ml) and ?-INF (100 ng/ml). Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.
    NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C. Detection with a fluorescent microscope: The cells were washed with Assay buffer and imaged using Nikon TiE microscope. (a) and (a) control cells (vehicle treated) (c) and (d) cells treated with LPS (200 ng/ml) and ?-INF (100 ng/ml). Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.

    Requested From: United States
    Date Requested: 3/26/2019
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