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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GST Activity Kit (Fluorometric) - LS-K55

Available for shipment within the USA only
Catalog
Size
Price
LS-K55-100
100 asy
$470

Available for shipment within the USA only

Product Description

Glutathione S-transferase (GST) is a family of enzymes that plays an important role in the detoxification of xenobiotics by formation of glutathione adducts via the thiol group. GST utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress, and is an important part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. The GST Fluorometric Activity Assay Kit provides a simple, fluorescence-based in vitro assay for detecting GST activity using a fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts directly with glutathione. The free form of MCB is almost non-fluorescent, whereas the dye fluoresces blue (Ex/Em = 380/461 nm) after reaction with glutathione catalyzed by GST. The change in fluorescence over time is thus allows for an easy direct measurement of the sample GST level. The kit can detect GST activity in crude cell lysate or purified protein fraction. The kit can also be used to detect or quantitate GST-tagged fusion recombinant protein. Detection sensitivity < 0.5 mU.

Specifications

Name
GST Activity Kit (Fluorometric)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Glutathione S-transferase (GST) levels in various biological sample types.
Target
Glutathione S-Transferase
Sample Type
Biological Fluids, Cell Culture Media, Cell Lysates, Plasma, Serum, Tissue Homogenates, Urine
Detection
Fluorometric (Ex/Em 380/461 nm)
Species Reactivity
Mammal
Kit Components
  • GST Assay Buffer
  • MCB Substrate
  • Glutathione (lyophilized)
  • GST Standard
  • GST Activity Assay Kit (Flurometric)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About Glutathione S-Transferase
    O18598

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    Customer Reviews (0)


    Images

    Assay Result

    Glutathione S-transferase (GST) Activity Assay Kit standard curve.
    Glutathione S-transferase (GST) Activity Assay Kit standard curve.

    Assay Result

    Glutathione S-transferase (GST) Activity Assay Kit standard curve.
    Glutathione S-transferase (GST) Activity Assay Kit standard curve.

    Assay Result

    Glutathione S-transferase (GST) Activity Assay Kit standard curve.
    Glutathione S-transferase (GST) Activity Assay Kit standard curve.

    Assay Result

    Glutathione S-transferase (GST) Activity Assay Kit standard curve.
    Glutathione S-transferase (GST) Activity Assay Kit standard curve.

    Requested From: United States
    Date Requested: 7/15/2019
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