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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-K612-100 100 asy $1,160 
Glucose Assay Kit - (a), (b) and (c) 2-DG uptake in human adipocytes, Hela Cells and 3T3-L1 cells respectively. Assays were performed following kit protocol. I = Insulin; P = Phloretin.

Glucose Uptake Assay Kit (Fluorometric) - LS-K612

Glucose Uptake Assay Kit (Fluorometric) - LS-K612

Available for shipment within the USA only
Description:
Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In The glucose uptake fluorometric assay kit, the accumulated 2-DG6P is enzymatically oxidized and coupled to The PicoProbe, which generates fluorescence in the presence of NADPH. This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.
Price
Catalog Number
$1,160 
LS-K612-100
100 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In The glucose uptake fluorometric assay kit, the accumulated 2-DG6P is enzymatically oxidized and coupled to The PicoProbe, which generates fluorescence in the presence of NADPH. This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.

Specifications

Name
Glucose Uptake Assay Kit (Fluorometric)
Type
Detection/Quantition
Usage
This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.
Target
Glucose
SampleType
Adherent Cell Culture, Suspension Cell Culture
Detection
Fluorometric (Ex/Em 535/587 nm)
Species Reactivity
Mammal
Components
  • Extraction Buffer
  • Neutralization Buffer
  • 2-Deoxyglucose (2-DG
  • 10 mM)
  • 2-DG Uptake Assay Buffer
  • Enzyme Mix (Lyophilized)
  • PicoProbe (in DMSO)
  • 2-DG6P Standard (Lyophilized)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Fast and convenient
    • Non-radioactive and highly sensitive
    • Simple procedure

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    Images

    Assay Result

    Glucose Assay Kit - (a), (b) and (c) 2-DG uptake in human adipocytes, Hela Cells and 3T3-L1 cells respectively. Assays were performed following kit protocol. I = Insulin; P = Phloretin.
    (a), (b) and (c) 2-DG uptake in human adipocytes, Hela Cells and 3T3-L1 cells respectively. Assays were performed following kit protocol. I = Insulin; P = Phloretin.

    Assay Result

    Glucose Assay Kit - (a), (b) and (c) 2-DG uptake in human adipocytes, Hela Cells and 3T3-L1 cells respectively. Assays were performed following kit protocol. I = Insulin; P = Phloretin.
    (a), (b) and (c) 2-DG uptake in human adipocytes, Hela Cells and 3T3-L1 cells respectively. Assays were performed following kit protocol. I = Insulin; P = Phloretin.

    Assay Result

    Glucose Assay Kit - (a), (b) and (c) 2-DG uptake in human adipocytes, Hela Cells and 3T3-L1 cells respectively. Assays were performed following kit protocol. I = Insulin; P = Phloretin.
    (a), (b) and (c) 2-DG uptake in human adipocytes, Hela Cells and 3T3-L1 cells respectively. Assays were performed following kit protocol. I = Insulin; P = Phloretin.

    Assay Result

    Glucose Assay Kit - (a), (b) and (c) 2-DG uptake in human adipocytes, Hela Cells and 3T3-L1 cells respectively. Assays were performed following kit protocol. I = Insulin; P = Phloretin.
    (a), (b) and (c) 2-DG uptake in human adipocytes, Hela Cells and 3T3-L1 cells respectively. Assays were performed following kit protocol. I = Insulin; P = Phloretin.

    Requested From: United States
    Date Requested: 2/24/2020