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Catalog Number Size Price
LS-K887-50 50 asy $1,160 
Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.
Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.
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Glucose Uptake Assay Kit (Cell-Based) - LS-K887

Glucose Uptake Assay Kit (Cell-Based) - LS-K887

Available for shipment within the USA only
Description:
Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolism and homeostasis. Cancer cells exhibit increased glucose uptake to support their high proliferation rate. This kit includes a fluorescent glucose analog, which just like glucose can be taken up by cells through glucose transporters. However, this glucose analog cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to the glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes phloretin, a natural phenol that inhibits glucose uptake. This easy-to-use non-radioactive kit allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin, growth factors, etc.
Price
Catalog Number
$1,160 
LS-K887-50
50 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolism and homeostasis. Cancer cells exhibit increased glucose uptake to support their high proliferation rate. This kit includes a fluorescent glucose analog, which just like glucose can be taken up by cells through glucose transporters. However, this glucose analog cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to the glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes phloretin, a natural phenol that inhibits glucose uptake. This easy-to-use non-radioactive kit allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin, growth factors, etc.

Specifications

Name
Glucose Uptake Assay Kit (Cell-Based)
Type
Detection/Quantition
Target
Glucose
SampleType
Adherent Cells, Suspension Cells
Detection
FACS
Components
  • Analysis Buffer (50X)
  • GluTracker Reagent (100X)
  • GluTracker Enhancer
  • Phloretin (100X)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Flow Cytometry, Microscopy (fluorescent)
    Equipment
    Flow cytometer, Fluorescence microscope
    Conditions
    Shipped +4°C Ice Packs, Store at -20°C. Avoid freeze-thaw cycles
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Easy-to-use
    • Images and accurately measures Glucose uptake in cultured cells in response to insulin, growth factors, etc.
    • non-radiometric

    Publications (0)

    Customer Reviews (0)

    Images

    Flow Cytometry

    Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).

    Fluorescence Microscopy

    Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.

    Flow Cytometry

    Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).

    Fluorescence Microscopy

    Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.

    Flow Cytometry

    Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).

    Fluorescence Microscopy

    Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.

    Flow Cytometry

    Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).

    Fluorescence Microscopy

    Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.

    Requested From: United States
    Date Requested: 2/24/2020