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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Glucose Uptake Assay Kit (Cell-Based) - LS-K887

Available for shipment within the USA only
Catalog
Size
Price
LS-K887-50
50 asy
$1,100

Available for shipment within the USA only

Product Description

LifeSpan Assay Kit

Specifications

Name
Glucose Uptake Assay Kit (Cell-Based)
Product Type
Detection/Quantition
Target
Glucose
Sample Type
Adherent Cells, Suspension Cells
Detection
FACS
Kit Components
  • Analysis Buffer (50X)
  • GluTracker Reagent (100X)
  • GluTracker Enhancer
  • Phloretin (100X)
  • (See Datasheet for specific volumes supplied)
  • Conditions
    Shipped +4°C Ice Packs, Store at Store at -20°C for up to 1 year.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

    Publications (0)


    Customer Reviews (0)


    Images

    Flow Cytometry

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).

    Fluorescence Microscopy

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.

    Flow Cytometry

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).

    Fluorescence Microscopy

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.

    Flow Cytometry

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).

    Fluorescence Microscopy

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.

    Flow Cytometry

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).

    Fluorescence Microscopy

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. A. Images of Jurkat cells obtained using fluorescence microscope (Top: treated with Phloretin; Bottom: without Phloretin treatment). B. Glucose Uptake in HeLa cells: HeLa cells showing the uptake of Glucose Tracker Reagent in the cytoplasm. Cells were stained with Glucose Tracker Reagent for 30 min. and fixed. Image was taken using a fluorescence microscope with a 60X objective lens.

    Requested From: United States
    Date Requested: 4/19/2019
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