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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-K110-100 100 asy $540 
Glucose Assay Kit - Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.

Glucose Assay Kit (Colorimetric/Fluorometric) - LS-K110

Glucose Assay Kit (Colorimetric/Fluorometric) - LS-K110

Available for shipment within the USA only
Description:
Glucose (C6H12O6; FW: 180.16) is a very important fuel source to generate universal energy molecules ATP. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes. The Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). Glucose Enzyme Mix specifically oxidizes glucose to generate a product which reacts with a dye to generate color (? = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the glucose amount. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit detects 1-10000 µM glucose samples.
Price
Catalog Number
$540 
LS-K110-100
100 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Glucose (C6H12O6; FW: 180.16) is a very important fuel source to generate universal energy molecules ATP. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes. The Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). Glucose Enzyme Mix specifically oxidizes glucose to generate a product which reacts with a dye to generate color (? = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the glucose amount. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit detects 1-10000 µM glucose samples.

Specifications

Name
Glucose Assay Kit (Colorimetric/Fluorometric)
Type
Detection/Quantition
Usage
This assay provides for the detection of Glucose in various biological samples.
Target
Glucose
SampleType
Biological Fluids, Growth Medium, Plasma, Serum, Tissue Culture Supernatants, Urine
Detection
Colorimetric (570 nm Absorbance), Fluorometric (Ex/Em 535/587 nm)
Species Reactivity
Mammal
Components
  • Glucose Assay Buffer
  • Glucose Probe (in DMSO)
  • Glucose Enzyme Mix (lyophilized)
  • Glucose Standard (100 nmol/ml)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric), Spectrophotometry (visible)
    Equipment
    Microplate fluorometer, Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Components stored at varying temperatures. See recommended storage details in product insert, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Fast and convenient
    • Provides all necessary buffers and reagents for an accurate assay of Glucose in various mammalian samples
    • Simple procedure
    • Takes ~40 minutes

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    Customer Reviews (0)

    Images

    Assay Result

    Glucose Assay Kit - Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.
    Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.

    Assay Result

    Glucose Assay Kit - Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.
    Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.

    Assay Result

    Glucose Assay Kit - Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.
    Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.

    Assay Result

    Glucose Assay Kit - Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.
    Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.

    Requested From: United States
    Date Requested: 2/24/2020