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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Fumarate Assay Kit (Colorimetric) - LS-K215

Available for shipment within the USA only
Catalog
Size
Price
LS-K215-100
100 tst
$360

Available for shipment within the USA only

Product Description

FUMARATE , or Fumaric Acid, is one of the key components in TCA cycle and is used by cells to form ATP. Human skin when exposed to sunlight will naturally produce fumaric acid. Fumarate is used as an additive by the food and beverage industries. Fumaric acid esters are also used to treat psoriasis. Increased urinary fumarate may be due to impaired Krebs cycle function, a defect in the enzyme fumarase or mitochondrial function. This fumarate assay kit is based on fumarase catalyzed hydration of fumarate to malate. The malate is then oxidized by malate dehydrogenase generating NADH which reduces a formazan (MTT) dye. The intensity of the product color, measured at 565 nm is proportional to the fumarate concentration in the sample.

Specifications

Name
Fumarate Assay Kit (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
For quantitative determination of fumarate and drug effects on its metabolism.
Target
Fumarate
Sample Type
Biological Samples, Food and Beverages
Detection
Colorimetric (565 nm Absorbance)
Kit Components
  • Assay Buffer
  • Enzyme A
  • NAD/MTT
  • Enzyme B
  • standard 20 mM Fumarate FMR Enzyme
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 6 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Requested From: United States
    Date Requested: 4/23/2019
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