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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-K747-50 50 asy $800 
Glucose Assay Kit - A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.

Direct Glucose Uptake Assay Kit (Fluorometric) - LS-K747

Direct Glucose Uptake Assay Kit (Fluorometric) - LS-K747

Available for shipment within the USA only
Description:
Glucose uptake is one of the key processes for cellular glucose metabolism. The study of glucose uptake can provide important information for understanding glucose metabolism and regulation in normal and disease development such as diabetes. The Glucose Uptake Assay kit is simple, ultra-sensitive and easy to use. A specific hexokinase inhibitor that inhibits hexokinase, the first enzyme metabolizing glucose in cells is used to arrest glucose consumption after its uptake. Glucose Uptake is measured by using a set of enzymatic reactions that specifically oxidize glucose producing intermediates that react with the OxiRed Probe generating a fluorescence signal (Ex/Em=535/587 nm). The fluorescence signal is directly proportional to the amount of glucose that has been taken up and accumulated inside the cells. Unlike other kits detecting glucose derivatives, this glucose uptake assay provides a direct, powerful tool for studying this process as well as for screening and characterization of drugs that regulate glucose uptake during normal and disease development
Price
Catalog Number
$800 
LS-K747-50
50 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Glucose uptake is one of the key processes for cellular glucose metabolism. The study of glucose uptake can provide important information for understanding glucose metabolism and regulation in normal and disease development such as diabetes. The Glucose Uptake Assay kit is simple, ultra-sensitive and easy to use. A specific hexokinase inhibitor that inhibits hexokinase, the first enzyme metabolizing glucose in cells is used to arrest glucose consumption after its uptake. Glucose Uptake is measured by using a set of enzymatic reactions that specifically oxidize glucose producing intermediates that react with the OxiRed Probe generating a fluorescence signal (Ex/Em=535/587 nm). The fluorescence signal is directly proportional to the amount of glucose that has been taken up and accumulated inside the cells. Unlike other kits detecting glucose derivatives, this glucose uptake assay provides a direct, powerful tool for studying this process as well as for screening and characterization of drugs that regulate glucose uptake during normal and disease development

Specifications

Name
Direct Glucose Uptake Assay Kit (Fluorometric)
Type
Detection/Quantition
Usage
Simple procedure. Fast and convenient. The assay is easy, non-radioactive, and highly sensitive
Target
Glucose
SampleType
Adherent Cells, Suspension Cells
Detection
Fluorometric (Ex/Em 535/587 nm)
Species Reactivity
Mammal
Components
  • Assay Buffer
  • OxiRed Probe (in DMSO)
  • Enzyme Mix
  • Hexokinase Inhibitor
  • Glucose Standard (100 mM)
  • Glucose (1 M Sterile)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Fast and convenient
    • Non-radioactive and highly sensitive
    • Simple procedure

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    Customer Reviews (0)

    Images

    Assay Result

    Glucose Assay Kit - A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.
    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.

    Assay Result

    Glucose Assay Kit - A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.
    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.

    Assay Result

    Glucose Assay Kit - A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.
    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.

    Assay Result

    Glucose Assay Kit - A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.
    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.

    Requested From: United States
    Date Requested: 2/21/2020