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Direct Glucose Uptake Assay Kit (Fluorometric) - LS-K747

Available for shipment within the USA only
Catalog
Size
Price
LS-K747-50
50 asy
$905

Available for shipment within the USA only

Product Description

Glucose uptake is one of the key processes for cellular glucose metabolism. The study of glucose uptake can provide important information for understanding glucose metabolism and regulation in normal and disease development such as diabetes. The Glucose Uptake Assay kit is simple, ultra-sensitive and easy to use. A specific hexokinase inhibitor that inhibits hexokinase, the first enzyme metabolizing glucose in cells is used to arrest glucose consumption after its uptake. Glucose Uptake is measured by using a set of enzymatic reactions that specifically oxidize glucose producing intermediates that react with the OxiRed Probe generating a fluorescence signal (Ex/Em=535/587 nm). The fluorescence signal is directly proportional to the amount of glucose that has been taken up and accumulated inside the cells. Unlike other kits detecting glucose derivatives, this glucose uptake assay provides a direct, powerful tool for studying this process as well as for screening and characterization of drugs that regulate glucose uptake during normal and disease development

Specifications

Name
Direct Glucose Uptake Assay Kit (Fluorometric)
Product Type
Detection/Quantition
Usage Summary
Simple procedure. Fast and convenient. The assay is easy, non-radioactive, and highly sensitive
Target
Glucose
Sample Type
Adherent Cells, Suspension Cells
Detection
Fluorometric (Ex/Em 535/587 nm)
Species Reactivity
Mammal
Kit Components
  • Assay Buffer
  • OxiRed Probe (in DMSO)
  • Enzyme Mix
  • Hexokinase Inhibitor
  • Glucose Standard (100 mM)
  • Glucose (1 M Sterile)
  • (See Datasheet for specific volumes supplied)
  • Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C for up to 1 year., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.
    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.

    Assay Result

    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.
    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.

    Assay Result

    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.
    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.

    Assay Result

    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.
    A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.

    Requested From: United States
    Date Requested: 6/16/2019
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