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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
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Crystal Violet Cell Cytotoxicity Assay Kit (Colorimetric) - LS-K425

Available for shipment within the USA only
Assay Kit
Catalog
Size
Price
LS-K425-1000
1000 asy
$385

Available for shipment within the USA only

Product Description

Crystal violet (CV) cell cytotoxicity assay is one of the common methods used to detect cell viability or drug cytotoxicity. CV is a triarylmethane dye that can bind to ribose type molecules such as DNA in nuclei. Normally, dead adherent cells will detach from cell culture plates and will be removed from viable cell propulation during washing steps. CV staining can be used to quantify the total DNA of the remaining population and thus determine cell viability. The CV staining is directly proportional to the cell biomass and can be measured at 570 nm. CV staining is a quick and versatile assay for screening cell viability under diverse stimulation or inhibition conditions. The CV cell cytotoxicity assay kit is simple, accurate, reproducible and sensitive. It also includes Doxorubicin as a positive control. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening

Specifications

Name
Crystal Violet Cell Cytotoxicity Assay Kit (Colorimetric)
Product Type
Cytotoxicity Assay
Usage Summary
This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays.This assay relies on the ability of crystal violet to bind ribose molecules (i.e. DNA) in viable cells.
Target
Cell Viability
Sample Type
Adherent cells
Detection
Colorimetric (570 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • Crystal Violet Staining Solution
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C for up to 1 year, 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Requested From: United States
    Date Requested: 9/15/2019