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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Chloride Assay Kit (Colorimetric) - LS-K548

Available for shipment within the USA only
Catalog
Size
Price
LS-K548-100
100 asy
$425

Available for shipment within the USA only

Product Description

Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter. The Chloride Assay Kit provides a quick, simple method for quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water. The assay is based upon the competition of Hg2+ and Fe2+ for TPTZ. The preferred Hg-TPTZ adduct exhibits no color. In the presence of Chloride, Hg2+ forms HgCL2 freeing up TPTZ which then binds the available Fe2+ giving a very intense absorbance with a ?max~ 620nm. The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.

Specifications

Name
Chloride Assay Kit (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.
Target
Chloride
Detection
Colorimetric (620 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • Chloride Reagent
  • Chloride Standard (10 µmol)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped Ambient, Store at Store at room temperature for up to 1 year., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    Chloride Standard Curve
    Chloride Standard Curve

    Assay Result

    Chloride Standard Curve
    Chloride Standard Curve

    Assay Result

    Chloride Standard Curve
    Chloride Standard Curve

    Assay Result

    Chloride Standard Curve
    Chloride Standard Curve

    Requested From: United States
    Date Requested: 4/23/2019
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