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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CETP Activity Kit II (Fluorometric) - LS-K74

Available for shipment within the USA only
Catalog
Size
Price
LS-K74-100
100 asy
$665

Available for shipment within the USA only

Product Description

Cholesteryl ester transfer protein (CETP) is a plasma protein that transfers a cholesteryl ester from HDL to LDL or VLDL in exchange for a triglyceride. HDL plays an important role in lipid metabolism and cardiovascular health. HDL transports cholesterol to the liver for excretion or to steroidogenic tissues for steroid synthesis. HDL also plays an important role in the reverse cholesterol transport pathway, removing cholesterol from lipid-filled macrophages, protecting against atherosclerosis. Because of this function, CETP is viewed as a target to increase HDL, with CETP inhibition an active area of research and several CETP inhibitors at various stages of drug development. LSBio's CETP Activity Fluorometric Assay Kit uses a self-quenched fluorescent neutral lipid that can be measured when transferred to an acceptor molecule. The fluorometric intensity is directly proportional to the amount of neutral lipid transfer. Rabbit serum is provided as a positive control and CETP inhibitor Torcetrapib is included for assay validation. This Assay Kit, in addition to measuring activity in serum, is also suitable for testing activity of recombinant protein.

Specifications

Name
CETP Activity Kit II (Fluorometric)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of CETP activity in various tissues including animal serum, plasma and recombinant protein. Includes Inhibitor control, Torcetrapib to validate the assay, and a Positive Control (rabbit serum).
Target
CETP
Sample Type
Animal Plasma, Animal Serum, Recombinant Protein
Detection
Fluorometric (Ex/Em 480/511 nm)
Species Reactivity
Mammal
Kit Components
  • CETP Assay Buffer
  • Donor Molecule (2.4 nmol/ml)
  • Acceptor Molecule
  • Positive Control (rabbit serum)
  • Inhibitor (Torcetrapib 1 mM)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped +4°C Ice Packs, Store at Store at 4°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About CETP
    P11597 NM_000078 NP_000069.2

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    Images

    Assay Result

    (a) Measurement of CETP activity of rabbit serum (1 µl), human plasma (1 µl) or recombinant human CETP (800 ng), (b) Inhibition of CETP activity from rabbit serum by Torcetrapi(b) The assay was run for 1 hr and the IC50 was determined to be 3.56 nM
    (a) Measurement of CETP activity of rabbit serum (1 µl), human plasma (1 µl) or recombinant human CETP (800 ng), (b) Inhibition of CETP activity from rabbit serum by Torcetrapi(b) The assay was run for 1 hr and the IC50 was determined to be 3.56 nM

    Assay Result

    (a) Measurement of CETP activity of rabbit serum (1 µl), human plasma (1 µl) or recombinant human CETP (800 ng), (b) Inhibition of CETP activity from rabbit serum by Torcetrapi(b) The assay was run for 1 hr and the IC50 was determined to be 3.56 nM
    (a) Measurement of CETP activity of rabbit serum (1 µl), human plasma (1 µl) or recombinant human CETP (800 ng), (b) Inhibition of CETP activity from rabbit serum by Torcetrapi(b) The assay was run for 1 hr and the IC50 was determined to be 3.56 nM

    Assay Result

    (a) Measurement of CETP activity of rabbit serum (1 µl), human plasma (1 µl) or recombinant human CETP (800 ng), (b) Inhibition of CETP activity from rabbit serum by Torcetrapi(b) The assay was run for 1 hr and the IC50 was determined to be 3.56 nM
    (a) Measurement of CETP activity of rabbit serum (1 µl), human plasma (1 µl) or recombinant human CETP (800 ng), (b) Inhibition of CETP activity from rabbit serum by Torcetrapi(b) The assay was run for 1 hr and the IC50 was determined to be 3.56 nM

    Assay Result

    (a) Measurement of CETP activity of rabbit serum (1 µl), human plasma (1 µl) or recombinant human CETP (800 ng), (b) Inhibition of CETP activity from rabbit serum by Torcetrapi(b) The assay was run for 1 hr and the IC50 was determined to be 3.56 nM
    (a) Measurement of CETP activity of rabbit serum (1 µl), human plasma (1 µl) or recombinant human CETP (800 ng), (b) Inhibition of CETP activity from rabbit serum by Torcetrapi(b) The assay was run for 1 hr and the IC50 was determined to be 3.56 nM

    Requested From: United States
    Date Requested: 5/20/2019
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