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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Caspase-5 Assay Kit (Colorimetric) - LS-K13

Available for shipment within the USA only
Catalog
Size
Price
LS-K13-25
25 asy
$255
LS-K13-100
100 asy
$470
LS-K13-200
200 asy
$655
LS-K13-400
400 asy
$1,050

Available for shipment within the USA only

Product Description

The Caspase-5 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspase-5 and related caspases that recognize the sequence WEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate WEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.

Specifications

Name
Caspase-5 Assay Kit (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity.
Target
CASP5 / Caspase 5
Sample Type
Cell Lysates, Tissue Homogenates
Detection
Colorimetric (400/405 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • WEHD-pNA (4 mM)
  • DTT (1M)
  • Dilution Buffer
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible), Cuvette
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 6-12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About CASP5 / Caspase 5
    P51878 NM_004347 NP_004338.3

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    Requested From: United States
    Date Requested: 2/18/2019
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