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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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2401 Fourth Avenue Suite 900
Seattle WA 98121

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Caspase-4 Assay Kit (Fluorometric) - LS-K66
Available for shipment within the USA only
Catalog
Size
Price
LS-K66-100
100 asy
$470
LS-K66-200
200 asy
$655
LS-K66-25
25 asy
$255
LS-K66-400
400 asy
$1,015

Available for shipment within the USA only

Product Description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase-4or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.

Specifications

Name
Caspase-4 Assay Kit (Fluorometric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection of Caspase-4 in various biological sample types. This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.
Target
CASP4 / Caspase 4
Sample Type
Cell Lysates, Tissue Homogenates
Detection
Fluorometric (Ex/Em 400/505 nm)
Species Reactivity
Mammal
Kit Components
  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • LEVD-AFC (1 mM)
  • DTT (1M)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer, Cuvette
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 6-12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About CASP4 / Caspase 4
    P49662 NM_001225 NP_001216.1

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    Requested From: United States
    Date Requested: 12/13/2018
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