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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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LSBio
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Catalog Number Size Price
LS-K66-100 100 asy $490 
LS-K66-200 200 asy $655 
LS-K66-25 25 asy $255 
LS-K66-400 400 asy $1,015 
CASP4 / Caspase 4 Assay Kit

Caspase-4 Assay Kit (Fluorometric) - LS-K66

Caspase-4 Assay Kit (Fluorometric) - LS-K66

Available for shipment within the USA only
Description:
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase-4or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.
Price
Catalog Number
$490 
LS-K66-100

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase-4or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.

Specifications

Name
Caspase-4 Assay Kit (Fluorometric)
Type
Detection/Quantition
Usage
This assay provides for the detection of Caspase-4 in various biological sample types. This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.
Target
CASP4 / Caspase 4
Synonyms
CASP4 | CASP-4 | Casp11 | Caspase 4 | Caspase-4 | Cysteine protease icerel-ii | ICE(rel)-II | ICE(rel)II | ICH-2 | Ich-3 | ICH2 | Protease ICH-2 | TX | Tx protease | Caspase 11 | ICEREL-II | Mih1/TX | Protease TX
SampleType
Cell Lysates, Tissue Homogenates
Detection
Fluorometric (Ex/Em 400/505 nm)
Species Reactivity
Mammal
Components
  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • LEVD-AFC (1 mM)
  • DTT (1M)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer, Fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles, 6-12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent Caspase activity
    • Fast and convenient
    • Simple one-step procedure
    • Takes only 1-2 hours

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    CASP4 / Caspase 4 Assay Kit

    CASP4 / Caspase 4 Assay Kit

    CASP4 / Caspase 4 Assay Kit

    CASP4 / Caspase 4 Assay Kit

    Requested From: United States
    Date Requested: 2/17/2020