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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-K5-25 25 asy $250 
LS-K5-100 100 asy $500 
LS-K5-200 200 asy $665 
LS-K5-400 400 asy $1,050 
CASP1 / Caspase 1 Assay Kit - Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.

Caspase-1/ICE Assay Kit (Colorimetric) - LS-K5

Caspase-1/ICE Assay Kit (Colorimetric) - LS-K5

Available for shipment within the USA only
Description:
Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.
Price
Catalog Number
$250 
LS-K5-25

Available for shipment within the USA only
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.

Specifications

Name
Caspase-1/ICE Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.
Target
CASP1 / Caspase 1
Synonyms
CASP1 | Caspase-1 | CASP-1 | CASP1 nirs variant 1 | Caspase 1 | ICE | ICE-like protease | IL-1 beta-converting enzyme | IL-1BC | IL1BC | IL1BCE | p45 | IL1B-convertase | Interleukin-1 beta convertase
SampleType
Cell Lysates, Tissue Homogenates
Detection
Colorimetric (400/405 nm Absorbance)
Species Reactivity
Mammal
Components
  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • YVAD-pNA (4 mM)
  • DTT (1 M)
  • Dilution Buffer
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible), Spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity
    • Fast and convenient
    • Simple one-step procedure
    • Takes 1-2 hours

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    Images

    Assay Result

    CASP1 / Caspase 1 Assay Kit - Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.
    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.

    Assay Result

    CASP1 / Caspase 1 Assay Kit - Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.
    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.

    Assay Result

    CASP1 / Caspase 1 Assay Kit - Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.
    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.

    Assay Result

    CASP1 / Caspase 1 Assay Kit - Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.
    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.

    Requested From: United States
    Date Requested: 4/5/2020