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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Reasearch Areas
Cell Cycle Pathways
Protein Family And Group
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LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
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Caspase-1/ICE Assay Kit (Colorimetric) - LS-K5

Available for shipment within the USA only
Catalog
Size
Price
LS-K5-25
25 asy
$250
LS-K5-100
100 asy
$470
LS-K5-200
200 asy
$665
LS-K5-400
400 asy
$1,050

Available for shipment within the USA only

Product Description

Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.

Specifications

Name
Caspase-1/ICE Assay Kit (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.
Target
CASP1 / Caspase 1
Sample Type
Cell Lysates, Tissue Homogenates
Detection
Colorimetric (400/405 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • YVAD-pNA (4 mM)
  • DTT (1 M)
  • Dilution Buffer
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible), Spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C, 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About CASP1 / Caspase 1
    P29466 NM_001223 NP_001214.1

    Publications (0)


    Customer Reviews (0)


    Images

    Assay Result

    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.
    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.

    Assay Result

    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.
    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.

    Assay Result

    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.
    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.

    Assay Result

    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.
    Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.

    Requested From: United States
    Date Requested: 9/21/2019