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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Calcium Detection Kit (Cell-Based) (Fluorometric) - LS-K483

Available for shipment within the USA only
Catalog
Size
Price
LS-K483-50
50 asy
$680

Available for shipment within the USA only

Product Description

Calcium is essential for all living organisms. Calcium sequestration and release into the cytoplasm function as a signal for many cellular processes. As an important second messenger, calcium is involved in many physiological processes such as nerve impulse transmission, muscle contraction, etc. Intracellular calcium is mainly stored in mitochondria and endoplasmic reticulum, and its concentration in cytosol is tightly regulated. Dysfunction of calcium homeostasis causes oxidative stress, cell death and pathogenesis of Alzheimer's disease. has developed a Calcium Staining Kit that contains membrane permeable, intracellular calcium probe. Upon cell entry, the calcium probe is hydrolyzed by intracellular esterases rendering it membrane impermeable. The cleaved calcium probe binds to intracellular calcium generating fluorescence. The intensity of fluorescence is directly proportional to intracellular calcium levels. A Positive Control reagent is also provided, and serves as experimental control, which elevates intracellular Calcium levels resulting in increased calcium staining. This easy-to-use, non-radioactive kit allows studying the regulation of calcium at the cellular level by using Fluorescence Microscopy and Flow Cytometry in cultured cells

Specifications

Name
Calcium Detection Kit (Cell-Based) (Fluorometric)
Product Type
Detection/Quantition
Usage Summary
The assay kit can selectively detect intracellular calcium content in suspension and/or adherent cells
Target
Calcium
Sample Type
Adherent Cell Cultures, Suspension Cell Cultures
Detection
Fluorometric, Flow Cytometer (FL1)
Species Reactivity
Eukaryote
Kit Components
  • Assay Buffer (50X)
  • Calcium Probe
  • Experimental Control (2000X)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Flow Cytometry, Microscopy (fluorescent)
    Equipment
    Flow cytometer, Fluorescence microscope
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C for up to 1 year., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Fluorescence Microscopy

    Calcium Staining in Jurkat and HeLa cells. 1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µl of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit’s protocol. Fluorescence microscope images of Positive Control Staining (basal calcium staining; left panel) and Cells treated with Experimental Control reagent (middle panel). Increased fluorescence induced by Positive Control reagent compared to the Basal Calcium levels confirms intracellular accumulation of Calcium in the cells.
    Calcium Staining in Jurkat and HeLa cells. 1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µl of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit’s protocol. Fluorescence microscope images of Positive Control Staining (basal calcium staining; left panel) and Cells treated with Experimental Control reagent (middle panel). Increased fluorescence induced by Positive Control reagent compared to the Basal Calcium levels confirms intracellular accumulation of Calcium in the cells.

    Fluorescence Microscopy

    Calcium Staining in Jurkat and HeLa cells. 1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µl of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit’s protocol. Fluorescence microscope images of Positive Control Staining (basal calcium staining; left panel) and Cells treated with Experimental Control reagent (middle panel). Increased fluorescence induced by Positive Control reagent compared to the Basal Calcium levels confirms intracellular accumulation of Calcium in the cells.
    Calcium Staining in Jurkat and HeLa cells. 1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µl of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit’s protocol. Fluorescence microscope images of Positive Control Staining (basal calcium staining; left panel) and Cells treated with Experimental Control reagent (middle panel). Increased fluorescence induced by Positive Control reagent compared to the Basal Calcium levels confirms intracellular accumulation of Calcium in the cells.

    Fluorescence Microscopy

    Calcium Staining in Jurkat and HeLa cells. 1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µl of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit’s protocol. Fluorescence microscope images of Positive Control Staining (basal calcium staining; left panel) and Cells treated with Experimental Control reagent (middle panel). Increased fluorescence induced by Positive Control reagent compared to the Basal Calcium levels confirms intracellular accumulation of Calcium in the cells.
    Calcium Staining in Jurkat and HeLa cells. 1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µl of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit’s protocol. Fluorescence microscope images of Positive Control Staining (basal calcium staining; left panel) and Cells treated with Experimental Control reagent (middle panel). Increased fluorescence induced by Positive Control reagent compared to the Basal Calcium levels confirms intracellular accumulation of Calcium in the cells.

    Fluorescence Microscopy

    Calcium Staining in Jurkat and HeLa cells. 1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µl of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit’s protocol. Fluorescence microscope images of Positive Control Staining (basal calcium staining; left panel) and Cells treated with Experimental Control reagent (middle panel). Increased fluorescence induced by Positive Control reagent compared to the Basal Calcium levels confirms intracellular accumulation of Calcium in the cells.
    Calcium Staining in Jurkat and HeLa cells. 1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µl of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit’s protocol. Fluorescence microscope images of Positive Control Staining (basal calcium staining; left panel) and Cells treated with Experimental Control reagent (middle panel). Increased fluorescence induced by Positive Control reagent compared to the Basal Calcium levels confirms intracellular accumulation of Calcium in the cells.

    Requested From: United States
    Date Requested: 4/23/2019
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