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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Calcium Assay Kit (Fluorometric) - LS-K474

Available for shipment within the USA only
Catalog
Size
Price
LS-K474-100
100 asy
$560

Available for shipment within the USA only

Product Description

Calcium (Ca, Atomic Number: 40), is an essential element for all living organisms. In human, Calcium ion (Ca2+) is released from bone to bloodstream and its level in serum is tightly controlled (8.4-11.4 mg/dL). Ca2+ acts as second messenger and plays many biological roles in signal transduction, gene expression, muscle contraction, synaptic plasticity and hormone release. Recent studies have found that dysregulation of calcium homeostasis leads to health problems, such as Alzheimer's Diseases and Parkinson's Disease, etc. The Calcium Fluorometric Assay kit provides an easy and quantitative method to measure Calcium in various biological fluids. Low concentration of N,N,N',N'-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN) is included in the assay and used to pre-treat samples to eliminate interference from other di-valent metals such as Zinc. The assay is based on the ability of our proprietary probe that binds specifically to Calcium cations generating a high fluorescence signal. The fluorescence is directly proportional to the amount of calcium, which can be quantified by measuring the generated fluorescence (Ex/Em= 500/530 nm) using a microplate reader. This assay kit provides an add-and-read, non-radioactive, and high-throughput adaptable assay suitable for Calcium detection. The assay is rapid (less than 10 min) and sensitive (Limit of Detection: <0.1 µM).

Specifications

Name
Calcium Assay Kit (Fluorometric)
Product Type
Detection/Quantition
Usage Summary
Measurement of Calcium concentration in biological samples. Analysis of the effects of effectors/synthetic agents/stimuli on Calcium signaling
Target
Calcium
Sample Type
Biological Fluids, Plasma, Serum
Detection
Fluorometric (Ex/Em 500/530 nm)
Species Reactivity
Eukaryote
Kit Components
  • Calcium Assay Buffer
  • Calcium Probe
  • TPEN (1000X)
  • Calcium Standard (500 mM)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C for up to 1 year., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Customer Reviews (0)


    Images

    Assay Result

    Measurement of Calcium in three Normal Human Pooled Serum samples (1:50 dilution; 2 µl for measurement).
    Measurement of Calcium in three Normal Human Pooled Serum samples (1:50 dilution; 2 µl for measurement).

    Assay Result

    Measurement of Calcium in three Normal Human Pooled Serum samples (1:50 dilution; 2 µl for measurement).
    Measurement of Calcium in three Normal Human Pooled Serum samples (1:50 dilution; 2 µl for measurement).

    Assay Result

    Measurement of Calcium in three Normal Human Pooled Serum samples (1:50 dilution; 2 µl for measurement).
    Measurement of Calcium in three Normal Human Pooled Serum samples (1:50 dilution; 2 µl for measurement).

    Assay Result

    Measurement of Calcium in three Normal Human Pooled Serum samples (1:50 dilution; 2 µl for measurement).
    Measurement of Calcium in three Normal Human Pooled Serum samples (1:50 dilution; 2 µl for measurement).

    Requested From: United States
    Date Requested: 4/23/2019
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