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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-K846-1000 1000 asy $370 
Cell Viability Assay Kit - Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min. at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.

Calcein AM Cell Viability Assay Kit (Fluorometric) - LS-K846

Calcein AM Cell Viability Assay Kit (Fluorometric) - LS-K846

Available for shipment within the USA only
Description:
Quantification of number of viable cells is an indispensable tool in Cell Biology research. LSBio's Calcein AM Cell Viability Assay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as low as 50 viable cells in less than 30 min. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
Price
Catalog Number
$370 
LS-K846-1000
1000 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Quantification of number of viable cells is an indispensable tool in Cell Biology research. LSBio's Calcein AM Cell Viability Assay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as low as 50 viable cells in less than 30 min. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.

Specifications

Name
Calcein AM Cell Viability Assay Kit (Fluorometric)
Type
Cell Viability
Target
Cell Viability
SampleType
Adherent Cells, Suspension Cells, Proliferating and Non-Proliferating Cells
Detection
Fluorometric (Ex/Em 485/530 nm)
Components
  • Calcein AM
  • Calcein Dilution Buffer
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped +4°C Ice Packs, Store at -20°C. Avoid freeze-thaw cycles
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Convenient and non-radiometric
    • Highly sensitive: Detects as low as 50 viable cells in less than 30 minute
    • High-throughput
    • Suitable for cell proliferation, cell viability, chemotaxis, cytotoxicity, and apoptosis

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    Customer Reviews (0)

    Images

    Assay Result

    Cell Viability Assay Kit - Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min. at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.
    Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min. at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.

    Assay Result

    Cell Viability Assay Kit - Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min. at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.
    Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min. at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.

    Assay Result

    Cell Viability Assay Kit - Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min. at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.
    Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min. at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.

    Assay Result

    Cell Viability Assay Kit - Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min. at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.
    Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min. at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.

    Requested From: United States
    Date Requested: 12/10/2019