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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Asparaginase Activity Kit (Colorimetric/Fluorometric) - LS-K41

Available for shipment within the USA only
Catalog
Size
Price
LS-K41-100
100 asy
$540

Available for shipment within the USA only

Product Description

Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on Dasparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs). The enzyme has been used to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products and to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). Metabolization of asparagine prevents acrylamide formation in fried foods (Maillard reaction). The enzyme's antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy. LSBio provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected colorimetrically (Lambda = 570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.

Specifications

Name
Asparaginase Activity Kit (Colorimetric/Fluorometric)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Asparaginase in various biological sample types. Assay can be used to detect Asparaginase enzyme's antineoplastic effects, based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine.
Target
Asparaginase
Sample Type
Biological Samples, Cell Lysates, Food and Beverages
Detection
Colorimetric (570 nm Absorbance), Fluorometric (Ex/Em 535/590 nm)
Kit Components
  • Asparaginase Assay Buffer
  • OxiRedGäó Probe (in DMSO)
  • Substrate Mix (lyophilized)
  • Aspartate Enzyme Mix (lyophilized)
  • Conversion Mix (lyophilized)
  • Positive Control (lyophilized)
  • Aspartate Standard (100 mM)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric), Spectrophotometry (visible)
    Equipment
    Microplate fluorometer, Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    Measure of Asparaginase Activity over time.
    Measure of Asparaginase Activity over time.

    Assay Result

    Measure of Asparaginase Activity over time.
    Measure of Asparaginase Activity over time.

    Assay Result

    Measure of Asparaginase Activity over time.
    Measure of Asparaginase Activity over time.

    Assay Result

    Measure of Asparaginase Activity over time.
    Measure of Asparaginase Activity over time.

    Requested From: United States
    Date Requested: 3/26/2019
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