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Catalog Number Size Price
LS-K242-100 100 Tests $460 
Ammonia Assay Kit

Ammonia Assay Kit (Colorimetric/Fluorometric) - LS-K242

Ammonia Assay Kit (Colorimetric/Fluorometric) - LS-K242

Available for shipment within the USA only
Description:
Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase). Simple, direct and automation-ready procedures for measuring NH3 are popular in research and drug discovery. This ammonia assay is designed to directly measure NH3 and NH4+. In this assay, NADH is converted to NAD+ in the presence of NH3, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at lambda ex/em = 360/450 nm is directly proportionate to the NH3 concentration in the sample.
Price
Catalog Number
$460
LS-K242-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase). Simple, direct and automation-ready procedures for measuring NH3 are popular in research and drug discovery. This ammonia assay is designed to directly measure NH3 and NH4+. In this assay, NADH is converted to NAD+ in the presence of NH3, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at lambda ex/em = 360/450 nm is directly proportionate to the NH3 concentration in the sample.

Specifications

Name
Ammonia Assay Kit (Colorimetric/Fluorometric)
Type
Detection/Quantition
Usage
For quantitative determination of ammonia/ammonium concentration.
Target
Ammonia
SampleType
Cell Cultures, Plasma, Serum, Saliva, Urine
Detection
Colorimetric (340 nm Absorbance), Fluorometric (Ex/Em 360/450 nm)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • Enzyme
  • Ketoglutarate
  • standard NADH Reagent: Dried
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (fluorometric), Spectrophotometry (visible)
Equipment
Microplate fluorometer, Microplate spectrophotometer (visible)
Conditions
Shipped -20°C Dry Ice, Store at -20°C, 3 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

Ammonia Assay Kit

Colorimetry

Ammonia Assay Kit

Colorimetry

Ammonia Assay Kit

Colorimetry

Ammonia Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/15/2024