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Catalog Number Size Price
LS-K307-250 250 Tests $470 
Alkaline Phosphatase Assay Kit

Alkaline Phosphatase (ALP) Assay Kit (Colorimetric) - LS-K307

Alkaline Phosphatase (ALP) Assay Kit (Colorimetric) - LS-K307

Available for shipment within the USA only
Description:
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery. This Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved Method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
Price
Catalog Number
$470
LS-K307-250

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery. This Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved Method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

Specifications

Name
Alkaline Phosphatase (ALP) Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate.
Target
Alkaline Phosphatase
SampleType
Plasma, Serum
Detection
Colorimetric (405 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • Mg Acetate (0.2 M)
  • pNPP Liquid (1 M)
  • Calibrator (Tartrazine)
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible), Spectrophotometer (visible)
Conditions
Shipped Ambient, Store at 4°C or -20°C, 12 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

Alkaline Phosphatase Assay Kit

Colorimetry

Alkaline Phosphatase Assay Kit

Colorimetry

Alkaline Phosphatase Assay Kit

Colorimetry

Alkaline Phosphatase Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/19/2024