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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Alanine Assay Kit (Colorimetric/Fluorometric) - LS-K39

Available for shipment within the USA only
Catalog
Size
Price
LS-K39-100
100 asy
$470

Available for shipment within the USA only

Product Description

Alanine is the 2nd most abundant of the 20 proteinogenic amino acids. Nonessential, it plays a key role in the glucose-alanine cycle between tissues and liver. In muscle and other tissues that degrade amino acids, amino groups are pooled as glutamate by transamination. Glutamate then transfers the amino group to pyruvate via alanine aminotransferase, forming alanine and a-ketoglutarate. The alanine is passed into the blood and transported to the liver. A reverse of the alanine aminotransferase reaction takes place in liver. Pyruvate can be used in gluconeogenesis, to form glucose which may return to other tissues through the circulatory system. There appears to be a correlation between alanine levels and higher blood pressure, energy intake, cholesterol levels, and body mass index. LSBio's Alanine Assay Kit provides a simple, sensitive detection method of alanine. In the kit, alanine is converted to pyruvate which is specifically detected leading to proportional color (?=570nm: 0-10 nmol) or fluorescence (Ex/Em 535/587nm: 0-1 nmol) development. Serum concentration: ~24-76>g/ml (~3-9 nmol/10 >l).

Specifications

Name
Alanine Assay Kit (Colorimetric/Fluorometric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection of Alanine in various biological sample types.
Target
Alanine
Sample Type
Biological Fluids, Plasma, Serum, Tissue Culture Supernatants, Urine
Detection
Colorimetric (570 nm Absorbance), Fluorescent (Ex/Em 535/587 nm)
Species Reactivity
Mammal
Kit Components
  • Alanine Assay Buffer
  • Alanine probe (in DMSO)
  • Alanine Converting Enzyme (lyophilized)
  • Alanine Development Mix (lyophilized)
  • Alanine Standard (10 -¦mol)(lyophilized)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric), Spectrophotometry (visible)
    Equipment
    Microplate fluorometer, Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Customer Reviews (0)


    Images

    Assay Result

    Alanine Colorimetric/Fluorometric Assay Kit standard curves.
    Alanine Colorimetric/Fluorometric Assay Kit standard curves.

    Assay Result

    Alanine Colorimetric/Fluorometric Assay Kit standard curves.
    Alanine Colorimetric/Fluorometric Assay Kit standard curves.

    Assay Result

    Alanine Colorimetric/Fluorometric Assay Kit standard curves.
    Alanine Colorimetric/Fluorometric Assay Kit standard curves.

    Assay Result

    Alanine Colorimetric/Fluorometric Assay Kit standard curves.
    Alanine Colorimetric/Fluorometric Assay Kit standard curves.

    Requested From: United States
    Date Requested: 3/26/2019
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