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Alanine Aminotransferase (ALT) Activity Kit (Color/Fluor) - LS-K38

Available for shipment within the USA only
Catalog
Size
Price
LS-K38-100
100 asy
$525

Available for shipment within the USA only

Product Description

Alanine aminotransferase (ALT) is a transaminase (EC 2.6.1.2) also called serum glutamic pyruvic transaminase (SGPT) or alanine transaminase. ALT is found in serum and in various body tissues, but is usually associated with the liver. It catalyzes the reaction: (a-ketoglutarate + alanine ? glutamate + pyruvate). It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In LSBio's ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ?-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (?max = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.

Specifications

Name
Alanine Aminotransferase (ALT) Activity Kit (Color/Fluor)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Alanine aminotransferase (ALT) in various biological sample types. Also suitable for high throughput analyses and the evaluation of the pentose phosphate pathway.
Target
GPT / Alanine Transaminase
Sample Type
Biological Fluids, Food and Beverages, Growth Medium, Plasma, Serum, Tissue Culture Supernatants, Urine
Detection
Colorimetric (570 nm Absorbance), Fluorescent (Ex/Em 535/587 nm)
Species Reactivity
Mammal
Kit Components
  • ALT Assay Buffer
  • Red Probe (in DMSO)
  • ALT Enzyme Mix (lyophilized)
  • ALT Substrate (lyophilized)
  • Pyruvate Standard (100 nmol/ul)
  • ALT positive control (lyophilized)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric), Spectrophotometry (visible)
    Equipment
    Microplate fluorometer, Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About GPT / Alanine Transaminase
    P24298 NM_005309 NP_005300.1

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    Customer Reviews (0)


    Images

    Assay Result

    (a) Measurement of alanine aminotransferase activity in Positive Control and (b) HepG2 Cells (10 µg) and Liver Lysate (15 µg).
    (a) Measurement of alanine aminotransferase activity in Positive Control and (b) HepG2 Cells (10 µg) and Liver Lysate (15 µg).

    Assay Result

    (a) Measurement of alanine aminotransferase activity in Positive Control and (b) HepG2 Cells (10 µg) and Liver Lysate (15 µg).
    (a) Measurement of alanine aminotransferase activity in Positive Control and (b) HepG2 Cells (10 µg) and Liver Lysate (15 µg).

    Assay Result

    (a) Measurement of alanine aminotransferase activity in Positive Control and (b) HepG2 Cells (10 µg) and Liver Lysate (15 µg).
    (a) Measurement of alanine aminotransferase activity in Positive Control and (b) HepG2 Cells (10 µg) and Liver Lysate (15 µg).

    Assay Result

    (a) Measurement of alanine aminotransferase activity in Positive Control and (b) HepG2 Cells (10 µg) and Liver Lysate (15 µg).
    (a) Measurement of alanine aminotransferase activity in Positive Control and (b) HepG2 Cells (10 µg) and Liver Lysate (15 µg).

    Requested From: United States
    Date Requested: 3/26/2019
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