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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ADP Assay Kit II (Colorimetric) - LS-K21

Available for shipment within the USA only
Catalog
Size
Price
LS-K21-100
100 asy
$565

Available for shipment within the USA only

Product Description

ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphorylases and kinases. ADP is stored in platelets and can be released to interact with variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. LSBio's ADP Assay Kit II is suitable for measuring ADP levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, ADP in the presence of ADP Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. ADP Assay Kit II is simple, fast and high throughput ready. It can detect less than 20 µM of ADP in samples.

Specifications

Name
ADP Assay Kit II (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection of ADP.
Target
ADP / Adenosine Diphosphate
Sample Type
Animal Tissues, Cell Cultures
Detection
Colorimetric (450 nm Absorbance)
Kit Components
  • ADP Assay Buffer
  • ADP Enzyme Mix (Lyophilized)
  • Developer (Lyophilized)
  • Probe (Lyophilized)
  • ADP Standard (Lyophilized)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    Measurement of ADP in fish liver (100 µg), fish egg (200 µg) & HeLa & Jurkat cell lysate (100 µg).
    Measurement of ADP in fish liver (100 µg), fish egg (200 µg) & HeLa & Jurkat cell lysate (100 µg).

    Assay Result

    Measurement of ADP in fish liver (100 µg), fish egg (200 µg) & HeLa & Jurkat cell lysate (100 µg).
    Measurement of ADP in fish liver (100 µg), fish egg (200 µg) & HeLa & Jurkat cell lysate (100 µg).

    Assay Result

    Measurement of ADP in fish liver (100 µg), fish egg (200 µg) & HeLa & Jurkat cell lysate (100 µg).
    Measurement of ADP in fish liver (100 µg), fish egg (200 µg) & HeLa & Jurkat cell lysate (100 µg).

    Assay Result

    Measurement of ADP in fish liver (100 µg), fish egg (200 µg) & HeLa & Jurkat cell lysate (100 µg).
    Measurement of ADP in fish liver (100 µg), fish egg (200 µg) & HeLa & Jurkat cell lysate (100 µg).

    Requested From: United States
    Date Requested: 3/26/2019
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