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Adenosine diphosphate (ADP) Assay Kit (Fluorometric) - LS-K202

Available for shipment within the USA only
Catalog
Size
Price
LS-K202-100
100 tst
$365

Available for shipment within the USA only

Product Description

Adenosine diphosphate (ADP) is the product of ATP dephosphorylation by ATPases. ADP can be converted back to ATP by ATP synthases. ADP levels regulate several enzymes involved in intermediary metabolism. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, since these assays require measurement of ATP in the sample before conversion of ADP to ATP, if the nascent ATP concentration is significantly higher than the ADP concentration, the ATP signal will drown out the ADP signal. This newly designed ADP Assay Kit provides a convenient fluorometric means to measure ADP level even in the presence of ATP. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate is then quantified by a fluorimetric Method (lexc/em = 530/590nm). The assay is simple, sensitive, stable, high-throughput adaptable and can detect as low as 0.1 µM ADP in biological samples.

Specifications

Name
Adenosine diphosphate (ADP) Assay Kit (Fluorometric)
Product Type
Detection/Quantition
Usage Summary
For quantitative determination of ADP determination in cells and other biological samples.
Target
ADP / Adenosine Diphosphate
Sample Type
Biological Samples
Detection
Fluorometric (Ex/Em 530/590 nm)
Kit Components
  • Reagent A
  • Enzyme
  • 10% TCA
  • Reagent B
  • standard
  • Neutralizer
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 6 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Fluorimetry

    Fluorimetry

    Fluorimetry

    Fluorimetry


    Requested From: United States
    Date Requested: 3/26/2019
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