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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Adenosine Diphosphate (ADP) Assay Kit (Chemiluminescent) - LS-K257

Available for shipment within the USA only
Catalog
Size
Price
LS-K257-100
100 tst
$315

Available for shipment within the USA only

Product Description

This ADP Assay Kit provides a rapid Method to measure ADP levels. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

Specifications

Name
Adenosine Diphosphate (ADP) Assay Kit (Chemiluminescent)
Product Type
Detection/Quantition
Usage Summary
For rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of drug effects on ADP metabolism.
Target
ADP / Adenosine Diphosphate
Sample Type
Cells
Detection
Luminometry
Kit Components
  • Assay Buffer
  • Substrate
  • Cosubstrate
  • ATP Enzyme
  • ADP Enzyme
  • standard 3 mM ADP
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (chemiluminescent)
    Equipment
    Microplate luminometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 6 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Luminometry


    Requested From: United States
    Date Requested: 3/26/2019
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