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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Adenosine Deaminase Activity (ADA) Assay Kit (Colorimetric) - LS-K2

Available for shipment within the USA only
Catalog
Size
Price
LS-K2-100
100 asy
$420

Available for shipment within the USA only

Product Description

Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2'-deoxyadenosine to inosine and 2'-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation. In LSBio's ADA Activity Assay, inosine formed from the breakdown of adenosine is converted to uric acid with ADA Convertor and ADA Developer. The uric acid is measured at OD 293 nm. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 1 mU recombinant Adenosine Deaminase.

Specifications

Name
Adenosine Deaminase Activity (ADA) Assay Kit (Colorimetric)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay measures total Activity of Adenosine Deaminase.
Target
ADA / Adenosine Deaminase
Sample Type
Cell Lysates, Nuclear Extracts, Purified Recombinant Protein, Tissue Homogenates
Detection
Colorimetric (293 nm Absorbance)
Kit Components
  • ADA Assay Buffer (10x)
  • ADA Convertor
  • ADA Developer
  • ADA Substrate
  • ADA Positive Control
  • Inosine Standard (10 mM)
  • U.V. transparent plate (96-well)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (UV)
    Equipment
    Microplate spectrophotometer (UV)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About ADA / Adenosine Deaminase
    P00813 NM_000022 NP_000013.2

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    Customer Reviews (0)


    Images

    Assay Result

    ADA specific activity in rat brain lysate and Jurkat cell lysate.
    ADA specific activity in rat brain lysate and Jurkat cell lysate.

    Assay Result

    ADA specific activity in rat brain lysate and Jurkat cell lysate.
    ADA specific activity in rat brain lysate and Jurkat cell lysate.

    Assay Result

    ADA specific activity in rat brain lysate and Jurkat cell lysate.
    ADA specific activity in rat brain lysate and Jurkat cell lysate.

    Assay Result

    ADA specific activity in rat brain lysate and Jurkat cell lysate.
    ADA specific activity in rat brain lysate and Jurkat cell lysate.

    Requested From: United States
    Date Requested: 4/19/2019
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