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Catalog Number Size Price
LS-K199-100 100 Tests $480 
ACO1 / Aconitase Assay Kit

Aconitase Assay Kit (Colorimetric) - LS-K199

Aconitase Assay Kit (Colorimetric) - LS-K199

Available for shipment within the USA only
Description:
ACONITASE (ACONITATE HYDRATASE) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich's ataxia and diabetes. This aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.
Price
Catalog Number
$480
LS-K199-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
ACONITASE (ACONITATE HYDRATASE) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich's ataxia and diabetes. This aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.

Specifications

Name
Aconitase Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.
Target
ACO1 / Aconitase
Synonyms
ACO1 | ACONS | Aconitase | Aconitase 1, soluble | Aconitate hydratase | Citrate hydro-lyase | IREB1 | IRE-BP 1 | IREBP | IRP1 | Ferritin repressor protein | IREBP1 | Iron regulatory protein 1
SampleType
Biological Samples
Detection
Colorimetric (565 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • Enzyme A
  • NADP/MTT
  • Enzyme B
  • standard Substrate
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible)
Conditions
Shipped -20°C Dry Ice, Store at -20°C, 6 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Images

Colorimetry

ACO1 / Aconitase Assay Kit

Colorimetry

ACO1 / Aconitase Assay Kit

Colorimetry

ACO1 / Aconitase Assay Kit

Colorimetry

ACO1 / Aconitase Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2024