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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Acid Phosphatase (AP) Activity Kit (Fluorometric)

Available for shipment within the USA only
Catalog
Size
Price
LS-K51-500
500 asy
$360

Available for shipment within the USA only

Product Description

Acid phosphatase (AP) dephosphorylates phosphate groups from the phosphate esters in acid conditions. Different forms of acid phosphatase are found in different organs, and their serum levels are used as a diagnosis for disease in the corresponding organs. For example, elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate resistant acid phosphatase levels may indicate the bone disease. LSBio's Acid Phosphatase Fluorometric Assay Kit uses non-fluorescent Methylumbelliferyl phosphate disodium (MUP) as the substrate which has Ex/Em=360/440 nm when dephosphorylated by AP. The kit is an ultra-sensitive, simple, direct and HTS- ready fluorometric assay designed to measure AP activity in serum and other bio-samples. The detection sensitivity is ~1 µU, more sensitive than colorimetric assays. The kit is suitable for both research and drug discovery.

Specifications

Name
Acid Phosphatase (AP) Activity Kit (Fluorometric)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Acid phosphatase (AP) in various biological sample types.
Target
Acid Phosphatase
Sample Type
Biological Fluids, Cell Culture Media, Cell Lysates, Plasma, Serum, Tissue Homogenates, Urine
Detection
Fluorometric (Ex/Em 360/440 nm)
Species Reactivity
Mammal
Kit Components
  • AP Assay Buffer
  • MUP Substrate (10 Tablets)
  • AP Enzyme
  • Stop Solution
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    Acid Phosphatase Activity Fluorometric Assay Kit standard curve.
    Acid Phosphatase Activity Fluorometric Assay Kit standard curve.

    Assay Result

    Acid Phosphatase Activity Fluorometric Assay Kit standard curve.
    Acid Phosphatase Activity Fluorometric Assay Kit standard curve.

    Assay Result

    Acid Phosphatase Activity Fluorometric Assay Kit standard curve.
    Acid Phosphatase Activity Fluorometric Assay Kit standard curve.

    Assay Result

    Acid Phosphatase Activity Fluorometric Assay Kit standard curve.
    Acid Phosphatase Activity Fluorometric Assay Kit standard curve.

    Requested From: United States
    Date Requested: 1/21/2019
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