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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

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Acid Phosphatase Activity Kit (Colorimetric)

Available for shipment within the USA only
Catalog
Size
Price
LS-K15-500
500 asy
$370

Available for shipment within the USA only

Product Description

Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. LSBio's Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (?max = 405 nm) when dephosphorylated by AP. The kit can detect µU acid phosphatase activity in samples.

Specifications

Name
Acid Phosphatase Activity Kit (Colorimetric)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Phosphate concentrations in various biological samples.
Target
Acid Phosphatase
Sample Type
Biological Fluids, Cell Culture Media, Cell Lysates, Plasma, Serum, Tissue Homogenates, Urine
Detection
Colorimetric (405 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • AP Assay Buffer
  • pNPP Substrate (10 Tablets)
  • AP Enzyme
  • Stop Solution
  • Acid Phosphatase Activity Assay Kit (Colorimetric)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    Measurement of Acid Phosphatase activity (40 minute assay).
    Measurement of Acid Phosphatase activity (40 minute assay).

    Assay Result

    Measurement of Acid Phosphatase activity (40 minute assay).
    Measurement of Acid Phosphatase activity (40 minute assay).

    Assay Result

    Measurement of Acid Phosphatase activity (40 minute assay).
    Measurement of Acid Phosphatase activity (40 minute assay).

    Assay Result

    Measurement of Acid Phosphatase activity (40 minute assay).
    Measurement of Acid Phosphatase activity (40 minute assay).

    Requested From: United States
    Date Requested: 1/23/2019
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