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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Acid Phosphatase (ACP) Assay Kit (Fluorometric)

Available for shipment within the USA only
Catalog
Size
Price
LS-K223-100
100 tst
$145

Available for shipment within the USA only

Product Description

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used. This non-radioactive, fluorimetric ACP assay is based on the cleavage of methylumbelliferyl phosphate. Methylumbelliferone becomes intensely fluorescent after addition of the stop reagent. The increase in fluorescence at 360/450 nm after addition of the stop reagent is directly proportional to the enzyme activity.

Specifications

Name
Acid Phosphatase (ACP) Assay Kit (Fluorometric)
Product Type
Detection/Quantition
Usage Summary
Acid Phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples.);
Target
Acid Phosphatase
Sample Type
Biological Samples, Serum
Detection
Fluorometric (Ex/Em 360/450 nm)
Kit Components
  • Assay Buffer
  • MUP Substrate
  • Stop Reagent
  • Standard
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped Ambient, Store at Store at -20°C., 6 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Fluorimetry

    Fluorimetry

    Fluorimetry

    Fluorimetry


    Requested From: United States
    Date Requested: 1/23/2019
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