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Catalog Number Size Price
LS-K223-100 100 Tests $220 
Acid Phosphatase Assay Kit

Acid Phosphatase (ACP) Assay Kit (Fluorometric) - LS-K223

Acid Phosphatase (ACP) Assay Kit (Fluorometric) - LS-K223

Available for shipment within the USA only
Description:
Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used. This non-radioactive, fluorimetric ACP assay is based on the cleavage of methylumbelliferyl phosphate. Methylumbelliferone becomes intensely fluorescent after addition of the stop reagent. The increase in fluorescence at 360/450 nm after addition of the stop reagent is directly proportional to the enzyme activity.
Price
Catalog Number
$220
LS-K223-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used. This non-radioactive, fluorimetric ACP assay is based on the cleavage of methylumbelliferyl phosphate. Methylumbelliferone becomes intensely fluorescent after addition of the stop reagent. The increase in fluorescence at 360/450 nm after addition of the stop reagent is directly proportional to the enzyme activity.

Specifications

Name
Acid Phosphatase (ACP) Assay Kit (Fluorometric)
Type
Detection/Quantition
Usage
Acid Phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples.);
Target
Acid Phosphatase
SampleType
Biological Samples, Serum
Detection
Fluorometric (Ex/Em 360/450 nm)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • MUP Substrate
  • Stop Reagent
  • Standard
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (fluorometric)
Equipment
Microplate fluorometer
Conditions
Shipped Ambient, Store at -20°C, 6 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Fluorimetry

Acid Phosphatase Assay Kit

Fluorimetry

Acid Phosphatase Assay Kit

Fluorimetry

Acid Phosphatase Assay Kit

Fluorimetry

Acid Phosphatase Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/5/2024