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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Acetylcholinesterase (AChE) Activity Kit (Color) - LS-K34

Available for shipment within the USA only
Catalog
Size
Price
LS-K34-100
100 asy
$540

Available for shipment within the USA only

Product Description

Acetylcholinesterase (AChE) is one of the most crucial enzymes associated with nerve response and function. AChE is a serine protease that hydrolyzes the neurotransmitter acetylcholine. Irreversible inhibition of AChE may lead to muscular paralysis, convulsions, bronchial constriction, and death by asphyxiation. This Acetylcholinesterase Activity Assay Kit provides a simple and sensitive method for continuous monitoring of enzymatic activity using colorimetry (OD 570 nm). In this assay, AChE converts acetylcholine substrate to choline, which is then oxidized by choline oxidase (CO) to produce an intermediate. The intermediate reacts with a highly specific probe to generate color. This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples.

Specifications

Name
Acetylcholinesterase (AChE) Activity Kit (Color)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Acetylcholinesterase (AChE) activity in various biological sample types, as well as for the screening of AChE inhibitors.
Target
ACHE / Acetylcholinesterase
Sample Type
Animal Tissue, Blood, Cell Culture, Human Serum, Plasma
Detection
Colorimetric (570 nm Absorbance)
Kit Components
  • AChE Assay Buffer
  • AChE Probe (DMSO)
  • AChE Substrate (lyophilized)
  • Choline Oxidase Enzyme Mix (lyophilized)
  • Choline Standard (lyophilized)
  • AChE Positive Control (lyophilized)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About ACHE / Acetylcholinesterase
    P22303 NM_015831 P22303

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    Images

    Assay Result

    AChE activity in serum (1 µl) & Positive Control (1 µl).
    AChE activity in serum (1 µl) & Positive Control (1 µl).

    Assay Result

    AChE activity in serum (1 µl) & Positive Control (1 µl).
    AChE activity in serum (1 µl) & Positive Control (1 µl).

    Assay Result

    AChE activity in serum (1 µl) & Positive Control (1 µl).
    AChE activity in serum (1 µl) & Positive Control (1 µl).

    Assay Result

    AChE activity in serum (1 µl) & Positive Control (1 µl).
    AChE activity in serum (1 µl) & Positive Control (1 µl).

    Requested From: United States
    Date Requested: 6/16/2019
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