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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-K751-100 100 asy $360 
Cell Viability Assay Kit - HepG2 Cells were cultured in 3D cell Culture BME Matrix for 21 days and spheroids were formed. Before dissociation, each spheroid was isolated from matrix and images were captured for sizing purposes (spheroid sizes i-iv, scale bar = 200 µm). Next, spheroids were dissociated, stained with Calcein AM, and measured following Viability Assay protocol (? RFU data are shown in bar graph).

3D Cell Culture HTS Cell Viability Assay Kit (Fluor) - LS-K751

3D Cell Culture HTS Cell Viability Assay Kit (Fluor) - LS-K751

Available for shipment within the USA only
Description:
Three dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fashion. 3D cell cultures improve the function, differentiation and viability of cells and recapitulate in vivo microenvironment compared to conventional 2D cell cultures. 3D matrices provide a physiologically relevant screening platform, by mimicking the in vivo responses, for many cell types including cancer and stem cells in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies. Quantification of number of viable cells is an indispensable tool in in vitro screening in these studies. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells, and has been widely used to assess cell viability and proliferation in Cell Biology research. However, with the use of 3D matrices, some proteases-based dissociation methods don't completely dissolve the matrices and cell aggregates, which could alter the result in quantitative in vitro assays such as viability assessment. The 3D Cell Culture Cell Viability Complete Assay Kit provides a standardized fluorometric method for sensitive quantification of viable cells that can detect as low as 50 viable cells in each well and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. Further, as a complete set, the kit comes with an optimized and gentle non-enzymatic dissociation solution for the recovery of viable and dead cells from spheroids in matrices and scaffolds. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for characterizing and screening cell viability, cytotoxicity and apoptosis.
Price
Catalog Number
$360 
LS-K751-100
100 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Three dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fashion. 3D cell cultures improve the function, differentiation and viability of cells and recapitulate in vivo microenvironment compared to conventional 2D cell cultures. 3D matrices provide a physiologically relevant screening platform, by mimicking the in vivo responses, for many cell types including cancer and stem cells in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies. Quantification of number of viable cells is an indispensable tool in in vitro screening in these studies. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells, and has been widely used to assess cell viability and proliferation in Cell Biology research. However, with the use of 3D matrices, some proteases-based dissociation methods don't completely dissolve the matrices and cell aggregates, which could alter the result in quantitative in vitro assays such as viability assessment. The 3D Cell Culture Cell Viability Complete Assay Kit provides a standardized fluorometric method for sensitive quantification of viable cells that can detect as low as 50 viable cells in each well and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. Further, as a complete set, the kit comes with an optimized and gentle non-enzymatic dissociation solution for the recovery of viable and dead cells from spheroids in matrices and scaffolds. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for characterizing and screening cell viability, cytotoxicity and apoptosis.

Specifications

Name
3D Cell Culture HTS Cell Viability Assay Kit (Fluor)
Type
3D Cell Culture
Usage
Matrix and spheroid dissociations from 3D cell culture for cell growth assessment. Measurement of cell viability in response to growth factors, cytokines, mitogens and nutrients. Analysis of cytotoxic/cytostatic compounds that affect cell growth and spheroid formation, such as anticancer drugs, toxic agents and other pharmaceuticals
Target
Cell Viability
SampleType
Non-proliferating Cells in 3D Culture Matrices, Non-proliferating Cells in 3D Culture Scaffolds, Proliferating Cells in 3D Culture Matrices, Proliferating Cells in 3D Culture Scaffolds
Detection
Fluorometric (Ex/Em 485/530 nm)
Species Reactivity
Mammal
Components
  • Matrix Dissociation Saline Solution
  • Viability Assay Buffer
  • Calcein AM
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Convenient and non-radioactive
    • Highly sensitive: Detects as low as 50 viable cells in less than 30 minute
    • High-throughput
    • Suitable for cell proliferation, cell viability, chemotaxis, cytotoxicity, and apoptosis

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    Customer Reviews (0)

    Images

    Assay Result

    Cell Viability Assay Kit - HepG2 Cells were cultured in 3D cell Culture BME Matrix for 21 days and spheroids were formed. Before dissociation, each spheroid was isolated from matrix and images were captured for sizing purposes (spheroid sizes i-iv, scale bar = 200 µm). Next, spheroids were dissociated, stained with Calcein AM, and measured following Viability Assay protocol (? RFU data are shown in bar graph).
    HepG2 Cells were cultured in 3D cell Culture BME Matrix for 21 days and spheroids were formed. Before dissociation, each spheroid was isolated from matrix and images were captured for sizing purposes (spheroid sizes i-iv, scale bar = 200 µm). Next, spheroids were dissociated, stained with Calcein AM, and measured following Viability Assay protocol (? RFU data are shown in bar graph).

    Assay Result

    Cell Viability Assay Kit - HepG2 Cells were cultured in 3D cell Culture BME Matrix for 21 days and spheroids were formed. Before dissociation, each spheroid was isolated from matrix and images were captured for sizing purposes (spheroid sizes i-iv, scale bar = 200 µm). Next, spheroids were dissociated, stained with Calcein AM, and measured following Viability Assay protocol (? RFU data are shown in bar graph).
    HepG2 Cells were cultured in 3D cell Culture BME Matrix for 21 days and spheroids were formed. Before dissociation, each spheroid was isolated from matrix and images were captured for sizing purposes (spheroid sizes i-iv, scale bar = 200 µm). Next, spheroids were dissociated, stained with Calcein AM, and measured following Viability Assay protocol (? RFU data are shown in bar graph).

    Assay Result

    Cell Viability Assay Kit - HepG2 Cells were cultured in 3D cell Culture BME Matrix for 21 days and spheroids were formed. Before dissociation, each spheroid was isolated from matrix and images were captured for sizing purposes (spheroid sizes i-iv, scale bar = 200 µm). Next, spheroids were dissociated, stained with Calcein AM, and measured following Viability Assay protocol (? RFU data are shown in bar graph).
    HepG2 Cells were cultured in 3D cell Culture BME Matrix for 21 days and spheroids were formed. Before dissociation, each spheroid was isolated from matrix and images were captured for sizing purposes (spheroid sizes i-iv, scale bar = 200 µm). Next, spheroids were dissociated, stained with Calcein AM, and measured following Viability Assay protocol (? RFU data are shown in bar graph).

    Assay Result

    Cell Viability Assay Kit - HepG2 Cells were cultured in 3D cell Culture BME Matrix for 21 days and spheroids were formed. Before dissociation, each spheroid was isolated from matrix and images were captured for sizing purposes (spheroid sizes i-iv, scale bar = 200 µm). Next, spheroids were dissociated, stained with Calcein AM, and measured following Viability Assay protocol (? RFU data are shown in bar graph).
    HepG2 Cells were cultured in 3D cell Culture BME Matrix for 21 days and spheroids were formed. Before dissociation, each spheroid was isolated from matrix and images were captured for sizing purposes (spheroid sizes i-iv, scale bar = 200 µm). Next, spheroids were dissociated, stained with Calcein AM, and measured following Viability Assay protocol (? RFU data are shown in bar graph).

    Requested From: United States
    Date Requested: 12/10/2019