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2-NBDG Glucose Uptake Assay Kit (Cell-Based) (Fluorometric) - LS-K620

Available for shipment within the USA only
Catalog
Size
Price
LS-K620-50
50 asy
$605

Available for shipment within the USA only

Product Description

Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolisms and homeostasis. Cancer cells exhibit increased glucose uptake to support their high proliferation rate. 2-NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose) is a fluorescent deoxyglucose analog that can be taken up by cells through glucose transporters. However, 2-NBDG cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes phloretin, a natural phenol that inhibits glucose uptake. This easy to use non-radioactive kit allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin, growth factors etc.

Specifications

Name
2-NBDG Glucose Uptake Assay Kit (Cell-Based) (Fluorometric)
Product Type
Detection/Quantition
Usage Summary
Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc. Dual-staining of glucose transporters and glucose uptake. Analysis of glucose metabolism and cell signaling in various cell types. Screening of anti-diabetic compounds
Target
Glucose
Sample Type
Adherent Cells, Suspension Cells
Detection
FACS (488 nm excitation laser), Fluorescent (excitation range 420-495 nm)
Kit Components
  • Analysis Buffer (50X)
  • 2-NBDG Reagent (100X)
  • Glucose Uptake Enhancer
  • Phloretin (100X)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Flow Cytometry, Microscopy (fluorescent)
    Equipment
    Flow cytometer, Fluorescence microscope
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C for up to 1 year., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Flow Cytometry

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were pretreated with or without 4 µl phloretin (1X concentration) for 45 min. After pretreatment, cells were washed and incubated with 2-NBDG Reagent, Glucose Uptake Enhancer, and the same concentration of phloretin for another 30 min. according to the kit’s protocol. Comparison of histograms from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were pretreated with or without 4 µl phloretin (1X concentration) for 45 min. After pretreatment, cells were washed and incubated with 2-NBDG Reagent, Glucose Uptake Enhancer, and the same concentration of phloretin for another 30 min. according to the kit’s protocol. Comparison of histograms from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).

    Flow Cytometry

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were pretreated with or without 4 µl phloretin (1X concentration) for 45 min. After pretreatment, cells were washed and incubated with 2-NBDG Reagent, Glucose Uptake Enhancer, and the same concentration of phloretin for another 30 min. according to the kit’s protocol. Comparison of histograms from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were pretreated with or without 4 µl phloretin (1X concentration) for 45 min. After pretreatment, cells were washed and incubated with 2-NBDG Reagent, Glucose Uptake Enhancer, and the same concentration of phloretin for another 30 min. according to the kit’s protocol. Comparison of histograms from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).

    Flow Cytometry

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were pretreated with or without 4 µl phloretin (1X concentration) for 45 min. After pretreatment, cells were washed and incubated with 2-NBDG Reagent, Glucose Uptake Enhancer, and the same concentration of phloretin for another 30 min. according to the kit’s protocol. Comparison of histograms from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were pretreated with or without 4 µl phloretin (1X concentration) for 45 min. After pretreatment, cells were washed and incubated with 2-NBDG Reagent, Glucose Uptake Enhancer, and the same concentration of phloretin for another 30 min. according to the kit’s protocol. Comparison of histograms from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).

    Flow Cytometry

    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were pretreated with or without 4 µl phloretin (1X concentration) for 45 min. After pretreatment, cells were washed and incubated with 2-NBDG Reagent, Glucose Uptake Enhancer, and the same concentration of phloretin for another 30 min. according to the kit’s protocol. Comparison of histograms from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).
    Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were pretreated with or without 4 µl phloretin (1X concentration) for 45 min. After pretreatment, cells were washed and incubated with 2-NBDG Reagent, Glucose Uptake Enhancer, and the same concentration of phloretin for another 30 min. according to the kit’s protocol. Comparison of histograms from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).

    Requested From: United States
    Date Requested: 4/19/2019
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