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LDH-Cytotoxicity Assay Kit (Colorimetric) LS-K1-400

Size: 400 Assays
Price: $500
USA Shipment Only
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Description: Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. The LDH-Cytotoxicity Assay Kit provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm. The LDH-cytotoxicity assay is sensitive, convenient, and precise, and is applicable to a variety of cytotoxicity studies. K1_AssayResult1
Jurkat cells were cultured in 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium. Light bar: Low control; Dark bar: High control.
LDH-Cytotoxicity Assay Kit (Colorimetric)
Lactate Dehydrogenase Cytotoxicity Assay Kit (Colorimetric)
LS-K1-400
400 Assays
Quantitative Assay Kit
This assay provides a convenient and non-radioactive alternative to the [3H]-thymidine and the [51Cr] release assays The assay is based on measurement of activity of lactate dehydrogenase (LDH) which is a stable enzyme normally found in the cytosol of all cells but rapidly releases into the supernatant upon damage of plasma membrane.
Cell cultures (adherent and suspension)
Colorimetric (500 nm Absorbance)
1 Hour
Mammalian
  • Catalyst, Lyophilized
  • Dye Solution
Shipped on gel packs, stored at -20°C, 12 month shelf life.
For research use only.
Data Sheet DataSheet    SDS Sheet SDS  

 


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