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Citrate Synthase Activity Assay Kit (Colorimetric) LS-K11-100

Size: 100 Assays
Price: $700
USA Shipment Only
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Description: Citrate Synthase (EC 2.3.3.1) is a key enzyme that is present in all living organisms. It catalyzes the conversion of acetyl-CoA and oxaloacetate into citrate and serves as a marker for intact mitochondria. A recent study showed that increased activity of mitochondrial Citrate Synthase is directly associated with metabolic and endocrine abnormalities such as obesity. In LSBio’s Citrate Synthase Activity Assay Kit, Citrate Synthase reacts with substrate mix to form an intermediate, which subsequently reacts with developer to generate the colored product. The rate of color development is proportional to the enzyme activity. The assay is simple, rapid and can detect Citrate Synthase activity less than 1 mU in a variety of samples. K11_AssayResult1.jpg
Citrate Synthase activity in Jurkat cell lysate (10 µg), rat liver lysate (20 µg), purified yeast mitochondria (4 µg) and CS Positive Control (2 µl).
Citrate Synthase Activity Assay Kit (Colorimetric)
LS-K11-100
100 Assays
Quantitative Assay Kit
Measurement of citrate synthase activity in various tissues/cells and analysis of intact mitochondria.
• Animal tissues: liver, heart, kidney, etc. • Cell culture: Adherent or suspension cells • Purified mitochondria
Colorimetric (412 nm Absorbance)
Less than 1 mU
  • CS Assay Buffer
  • CS Substrate Mix (Lyophilized)
  • CS Developer (Lyophilized)
  • GSH Standard (reduced) (Lyophilized)
  • CS Positive Control (Lyophilized)
Shipped on gel packs, stored at -20°C, 12 month shelf life.
For research use only.
Data Sheet DataSheet    SDS Sheet SDS  

 


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