Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: The Caspase-5 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspase-5 and related caspases that recognize the sequence WEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate WEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity.
Cell and tissue lysates
Colorimetric (400 or 405 nm Absorbance)
Cell Lysis Buffer
2X Reaction Buffer
WEHD-pNA (4 mM)
DTT (1 M)
Shipped on gel packs, stored at -20°C, 6-12 month shelf life.